Challenges in detecting coagulase positive Staphylococci isolated from skin infections of dogs in basic clinical laboratory
dc.contributor.author | Ananda Chitra, M | |
dc.contributor.author | Ramachandran, Maya | |
dc.contributor.author | Varughese, Hridya Susan | |
dc.contributor.author | TANUVAS | |
dc.date.accessioned | 2020-03-06T09:16:26Z | |
dc.date.available | 2020-03-06T09:16:26Z | |
dc.date.issued | 2020-02 | |
dc.description | TNV_20thMVC_PP_Feb-2020_OP61 | en_US |
dc.description.abstract | The coagulase test is one way to differentiate the highly pathogenic Staphylococcus from the other less pathogenic staphylococcal species. Out of seven coagulase-positive staphylococci (CoPS), S. pseudintermedius, S. schleiferi subsp. coagulans and S. aureus are present in dogs. In the present study, we have tested the suitability of the commercially available chromogenic media and latex agglutination test kit to detect coagulase positive staphylococci isolated from skin infections of dogs. In HiCrome Staph agar, S.aureus produces greenish blue colour colony. However, SP isolates and some SSC isolates were also produced similar greenish blue colour colonies. Commonly used and commercially available latex agglutination test for diagnosis of S. aureus was tested for SP and SSC isolates. Both isolates clearly produced agglutination of latex beads as that of S. aureus. In HiCrome UTI agar, S. aureus isolates produce golden yellow colour colonies. In 18-24 hours of incubation of most of the SP and few SSC isolates also produce similar yellow colour colonies but most of these isolates later turned into pink purple colour colonies. All these results suggest that chromogenic media and latex agglutination test can not distinguish these coagulase positive staphylococci which are commonly preferred in basic clinical laboratory. Hence, molecular diagnostic test is required to clearly detect each organism and development of any rapid molecular diagnostic test will aid clinicians/veterinarians to treat dog with skin infections appropriately. | en_US |
dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810144349 | |
dc.keywords | 20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVAS | en_US |
dc.language.iso | en | en_US |
dc.pages | 1 | en_US |
dc.subject | Veterinary Science | en_US |
dc.title | Challenges in detecting coagulase positive Staphylococci isolated from skin infections of dogs in basic clinical laboratory | en_US |
dc.title.alternative | National Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safety | en_US |
dc.type | Presentation | en_US |
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