Challenges in detecting coagulase positive Staphylococci isolated from skin infections of dogs in basic clinical laboratory

Simple item page
dc.contributor.authorAnanda Chitra, M
dc.contributor.authorRamachandran, Maya
dc.contributor.authorVarughese, Hridya Susan
dc.contributor.authorTANUVAS
dc.date.accessioned2020-03-06T09:16:26Z
dc.date.available2020-03-06T09:16:26Z
dc.date.issued2020-02
dc.descriptionTNV_20thMVC_PP_Feb-2020_OP61en_US
dc.description.abstractThe coagulase test is one way to differentiate the highly pathogenic Staphylococcus from the other less pathogenic staphylococcal species. Out of seven coagulase-positive staphylococci (CoPS), S. pseudintermedius, S. schleiferi subsp. coagulans and S. aureus are present in dogs. In the present study, we have tested the suitability of the commercially available chromogenic media and latex agglutination test kit to detect coagulase positive staphylococci isolated from skin infections of dogs. In HiCrome Staph agar, S.aureus produces greenish blue colour colony. However, SP isolates and some SSC isolates were also produced similar greenish blue colour colonies. Commonly used and commercially available latex agglutination test for diagnosis of S. aureus was tested for SP and SSC isolates. Both isolates clearly produced agglutination of latex beads as that of S. aureus. In HiCrome UTI agar, S. aureus isolates produce golden yellow colour colonies. In 18-24 hours of incubation of most of the SP and few SSC isolates also produce similar yellow colour colonies but most of these isolates later turned into pink purple colour colonies. All these results suggest that chromogenic media and latex agglutination test can not distinguish these coagulase positive staphylococci which are commonly preferred in basic clinical laboratory. Hence, molecular diagnostic test is required to clearly detect each organism and development of any rapid molecular diagnostic test will aid clinicians/veterinarians to treat dog with skin infections appropriately.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810144349
dc.keywords20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVASen_US
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleChallenges in detecting coagulase positive Staphylococci isolated from skin infections of dogs in basic clinical laboratoryen_US
dc.title.alternativeNational Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safetyen_US
dc.typePresentationen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
TNV_20thMVC_PP_Feb-2020_OP61.pdf
Size:
110 KB
Format:
Adobe Portable Document Format
Description:
TNV_20thMVC_PP_Feb-2020_OP61
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Poster Presentations