Challenges in detecting coagulase positive Staphylococci isolated from skin infections of dogs in basic clinical laboratory
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Date
2020-02
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Abstract
The coagulase test is one way to differentiate the highly pathogenic Staphylococcus from the other
less pathogenic staphylococcal species. Out of seven coagulase-positive staphylococci (CoPS), S.
pseudintermedius, S. schleiferi subsp. coagulans and S. aureus are present in dogs. In the present study, we
have tested the suitability of the commercially available chromogenic media and latex agglutination test kit to
detect coagulase positive staphylococci isolated from skin infections of dogs. In HiCrome Staph agar, S.aureus
produces greenish blue colour colony. However, SP isolates and some SSC isolates were also produced
similar greenish blue colour colonies. Commonly used and commercially available latex agglutination test
for diagnosis of S. aureus was tested for SP and SSC isolates. Both isolates clearly produced agglutination
of latex beads as that of S. aureus. In HiCrome UTI agar, S. aureus isolates produce golden yellow colour
colonies. In 18-24 hours of incubation of most of the SP and few SSC isolates also produce similar yellow
colour colonies but most of these isolates later turned into pink purple colour colonies. All these results
suggest that chromogenic media and latex agglutination test can not distinguish these coagulase positive
staphylococci which are commonly preferred in basic clinical laboratory. Hence, molecular diagnostic test
is required to clearly detect each organism and development of any rapid molecular diagnostic test will aid
clinicians/veterinarians to treat dog with skin infections appropriately.
Description
TNV_20thMVC_PP_Feb-2020_OP61
Keywords
Veterinary Science