GELATIN ZYMOGRAPHY OF MATRIX METALLOPROTEINASES IN NEAT SEMEN OF CATTLE AND BUFFALOES

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dc.contributor.authorSathyabama, T
dc.contributor.authorSabir Salim
dc.contributor.authorAdarsh Ram, et al.
dc.contributor.authorTANUVAS
dc.date.accessioned2020-02-28T11:57:38Z
dc.date.available2020-02-28T11:57:38Z
dc.date.issued2019-12
dc.descriptionTNV_35thVCRI_CA_Dec-2019_RBTO-26en_US
dc.description.abstractThe present study was carried out to ascertain the presence of Matrix Metalloproteinases (MMPs) enzyme activity in neat semen samples of Murrah breed of buffalo by gelatin zymography and the results were compared with the neat semen samples of exotic cattle breed. In gelatin zymography, the presence of one band at 92 kDa of MMP-9 and 72 kDa of MMP-2 were observed in all the samples. The catalytically active lower molecular weight MMPs were observed around 72 kDa. The activity of MMP-2 was 3-4 times higher than that of MMP-9.The activity of MMP-9 (92KDa) and MMP-2 (45KDa) were observed in Murrah buffalo which was absent in the exotic cattle breed. It was concluded that the overall activity of MMPs was at least two fold greater in buffalo breed compared with the activity of MMPs in Holstein Friesian semen samples. Further, it was inferred that purification and characterization of the species specific enzymes and their effect on semen samples could be correlated to use them as an additive in frozen semen samples, as MMP played extensive role in various physiological processes viz., fertilization, ovulation, milk secretion.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810143954
dc.keywords35th Annual Convention of ISSAR, VCRI, Namakkalen_US
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleGELATIN ZYMOGRAPHY OF MATRIX METALLOPROTEINASES IN NEAT SEMEN OF CATTLE AND BUFFALOESen_US
dc.title.alternativeInternational Symposium on Global perspectives to enhance livestock fertility through modern reproductive techniques for doubling farmer's Incomeen_US
dc.typeOtheren_US
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