GELATIN ZYMOGRAPHY OF MATRIX METALLOPROTEINASES IN NEAT SEMEN OF CATTLE AND BUFFALOES
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Date
2019-12
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Abstract
The present study was carried out to ascertain the presence of Matrix Metalloproteinases (MMPs)
enzyme activity in neat semen samples of Murrah breed of buffalo by gelatin zymography and the results were
compared with the neat semen samples of exotic cattle breed. In gelatin zymography, the presence of one band
at 92 kDa of MMP-9 and 72 kDa of MMP-2 were observed in all the samples. The catalytically active lower
molecular weight MMPs were observed around 72 kDa. The activity of MMP-2 was 3-4 times higher than that
of MMP-9.The activity of MMP-9 (92KDa) and MMP-2 (45KDa) were observed in Murrah buffalo which
was absent in the exotic cattle breed. It was concluded that the overall activity of MMPs was at least two fold
greater in buffalo breed compared with the activity of MMPs in Holstein Friesian semen samples. Further, it
was inferred that purification and characterization of the species specific enzymes and their effect on semen
samples could be correlated to use them as an additive in frozen semen samples, as MMP played extensive
role in various physiological processes viz., fertilization, ovulation, milk secretion.
Description
TNV_35thVCRI_CA_Dec-2019_RBTO-26
Keywords
Veterinary Science