Point of Care Tuberculosis Sero-Diagnosis Kit for Wild Animals: Combination of Proteins for Improving the Diagnostic Sensitivity and Specificity

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dc.contributor.authorVeerasami, Maroudam
dc.contributor.authorVenkataraman, K
dc.contributor.authorKaruppannan, Chitra, et al.
dc.contributor.authorTANUVAS
dc.date.accessioned2020-02-10T12:09:33Z
dc.date.available2020-02-10T12:09:33Z
dc.date.issued2018-01
dc.descriptionTNV_IJM_2018_58(1)81-92en_US
dc.description.abstractTuberculosis is a significant problem globally for domestic animals as well as captive and free ranging wild life. Rapid point of care (POC) serology kits are well suited for the diagnosis of TB in wild animals. However, wild animals are invariably exposed to environmental nonpathogenic mycobacterium species with the development of cross reacting antibodies. In the present study, POC TB diagnosis kit was developed using a combination of pathogenic Mycobacteria specific recombinant antigens and purified protein derivatives of pathogenic and nonpathogenic Mycobacteria. To benchmark the TB antibody detection kit, particularly in respect to specificity which could not be determined in wildlife due to the lack of samples from confirmed uninfected animals, we first tested well-characterized sera from 100 M. bovis infected and 100 uninfected cattle. Then we investigated the kit’s performance using sera samples from wildlife, namely Sloth Bears (n = 74), Elephants (n = 9), Cervidae (n = 14), Felidae (n = 21), Cape buffalo (n = 2), Wild bear (n = 1) and Wild dog (n = 1).In cattle, a sensitivity of 81% and a specificity of 90% were obtained. The diagnostic sensitivity of the kit was 94% when the kit was tested using known TB positive sloth bear sera samples. 47.4% of the in-contact sloth bears turned seropositive using the rapid POC TB diagnostic kit. Seropositivity in other wild animals was 25% when the sera samples were tested using the kit. A point of care TB sero-diagnostic kit with the combination of proteins was developed and the kit was validated using the sera samples of wild animals.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810142842
dc.keywordsMycobacteria, POC, Rapid test, Tuberculosis, Wild TB diagnostic kiten_US
dc.language.isoenen_US
dc.pages81-92en_US
dc.relation.ispartofseries;1
dc.subjectVeterinary Scienceen_US
dc.titlePoint of Care Tuberculosis Sero-Diagnosis Kit for Wild Animals: Combination of Proteins for Improving the Diagnostic Sensitivity and Specificityen_US
dc.title.alternativeIndian Journal Microbiolen_US
dc.typeArticleen_US
dc.volume58en_US
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