Point of Care Tuberculosis Sero-Diagnosis Kit for Wild Animals: Combination of Proteins for Improving the Diagnostic Sensitivity and Specificity
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Date
2018-01
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Abstract
Tuberculosis is a significant problem globally
for domestic animals as well as captive and free ranging
wild life. Rapid point of care (POC) serology kits are well
suited for the diagnosis of TB in wild animals. However,
wild animals are invariably exposed to environmental nonpathogenic
mycobacterium species with the development
of cross reacting antibodies. In the present study, POC TB
diagnosis kit was developed using a combination of
pathogenic Mycobacteria specific recombinant antigens
and purified protein derivatives of pathogenic and nonpathogenic
Mycobacteria. To benchmark the TB antibody
detection kit, particularly in respect to specificity which
could not be determined in wildlife due to the lack of
samples from confirmed uninfected animals, we first tested
well-characterized sera from 100 M. bovis infected and 100
uninfected cattle. Then we investigated the kit’s performance
using sera samples from wildlife, namely Sloth
Bears (n = 74), Elephants (n = 9), Cervidae (n = 14),
Felidae (n = 21), Cape buffalo (n = 2), Wild bear (n = 1)
and Wild dog (n = 1).In cattle, a sensitivity of 81% and a
specificity of 90% were obtained. The diagnostic sensitivity
of the kit was 94% when the kit was tested using
known TB positive sloth bear sera samples. 47.4% of the
in-contact sloth bears turned seropositive using the rapid
POC TB diagnostic kit. Seropositivity in other wild animals
was 25% when the sera samples were tested using the
kit. A point of care TB sero-diagnostic kit with the combination
of proteins was developed and the kit was validated
using the sera samples of wild animals.
Description
TNV_IJM_2018_58(1)81-92
Keywords
Veterinary Science