Virulence genes and enterobacterial repetitive intergenic consensus region (ERIC) profiling reveals highly diverse genetic population among avian strains of Pasteurella multocida
dc.contributor.author | Karthik, Kumaragurubaran | |
dc.contributor.author | Prasanna Devi, Rajan | |
dc.contributor.author | Ananda Chitra, Murugesan, et al., | |
dc.contributor.author | TANUVAS | |
dc.date.accessioned | 2021-12-24T11:23:30Z | |
dc.date.available | 2021-12-24T11:23:30Z | |
dc.date.issued | 2021 | |
dc.description | TNV_MP_2021_161(1) | en_US |
dc.description.abstract | Pasteurella multocida is a multispecies pathogen with certain host specific capsular types but interspecies transmission cannot be overlooked. Knowing the diversity of P. multocida in a geographical location is essential to formulate a vaccination programme. Diversity among the P. multocida isolates from different avian species recovered in the state of Tamil Nadu, India was studied using enterobacterial repetitive intergenic consensus region (ERIC)-PCR and virulence gene profiling (VP). Capsular typing revealed that 44 (97.78%) strains belonged to capsular type A while only one (2.22%) strain belonged to capsular type B. ERIC-PCR analysis showed eight different clusters and four individual strains. The index of discrimination (D value) was found to be 0.8899. Virulence profiling showed that genes fimA, pfhA, hsf-2 and pmHAS were found in 100% of the strains while ompH, omp87, ompA, plpB, sodA, sodC, ptfA, hsf-1, exbB, fur, hgbA and hgbB were found in ≥90% of the strains. Dermonecrotoxin gene toxA was present only in 4.44% of the strains, while nanH in 68.89% and nanB in 88.89% of the strains. One strain each from turkey and Guinea fowl had toxA gene. Correlation analysis revealed a positive correlation between ptfA and hgbA gene, exbB and fur gene, ptfA and sodC gene, exbB and hsf-1 gene, ompA and ompH gene. Majority of duck strains clustered together both in ERIC and virulence gene profiles. Turkey strains were highly diverse with different VPs and ERIC-PCR patterns. | en_US |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810179873 | |
dc.keywords | Pasteurella multocida ERIC-PCR Virulence gene profiling Avian species Turkey Correlation analysis | en_US |
dc.language.iso | English | en_US |
dc.pages | 1 | en_US |
dc.subject | Veterinary Science | en_US |
dc.title | Virulence genes and enterobacterial repetitive intergenic consensus region (ERIC) profiling reveals highly diverse genetic population among avian strains of Pasteurella multocida | en_US |
dc.title.alternative | Microbial Pathogenesis | en_US |
dc.type | Article | en_US |
dc.volume | 161 | en_US |
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