Virulence genes and enterobacterial repetitive intergenic consensus region (ERIC) profiling reveals highly diverse genetic population among avian strains of Pasteurella multocida
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Date
2021
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Abstract
Pasteurella multocida is a multispecies pathogen with certain host specific capsular types but interspecies transmission
cannot be overlooked. Knowing the diversity of P. multocida in a geographical location is essential to
formulate a vaccination programme. Diversity among the P. multocida isolates from different avian species
recovered in the state of Tamil Nadu, India was studied using enterobacterial repetitive intergenic consensus
region (ERIC)-PCR and virulence gene profiling (VP). Capsular typing revealed that 44 (97.78%) strains belonged
to capsular type A while only one (2.22%) strain belonged to capsular type B. ERIC-PCR analysis showed eight
different clusters and four individual strains. The index of discrimination (D value) was found to be 0.8899.
Virulence profiling showed that genes fimA, pfhA, hsf-2 and pmHAS were found in 100% of the strains while
ompH, omp87, ompA, plpB, sodA, sodC, ptfA, hsf-1, exbB, fur, hgbA and hgbB were found in ≥90% of the strains.
Dermonecrotoxin gene toxA was present only in 4.44% of the strains, while nanH in 68.89% and nanB in 88.89%
of the strains. One strain each from turkey and Guinea fowl had toxA gene. Correlation analysis revealed a
positive correlation between ptfA and hgbA gene, exbB and fur gene, ptfA and sodC gene, exbB and hsf-1 gene,
ompA and ompH gene. Majority of duck strains clustered together both in ERIC and virulence gene profiles.
Turkey strains were highly diverse with different VPs and ERIC-PCR patterns.
Description
TNV_MP_2021_161(1)
Keywords
Veterinary Science