ISOLATION AND MOLECULAR CHARACTERIZATION OF ANTIMICROBIAL RESISTANT Escherichia coli IN PORK PRODUCTION CHAIN IN AND AROUND BENGALURU
| dc.contributor.advisor | SHRIKRISHNA ISLOOR | |
| dc.contributor.author | SHIVAKUMARA, R. | |
| dc.date.accessioned | 2024-01-09T11:14:11Z | |
| dc.date.available | 2024-01-09T11:14:11Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | The present investigation was carried out to evaluate the occurrence of AMR E. coli in pork production chain in and around Bengaluru. Initial survey indicated that majority of the pig farmers were small and marginal farmers (66%) and kitchen / hotel waste was the major source of feed. The major antibiotics used were tetracycline followed by enrofloxacin and sulphonamides (Co-trimazole) and antibiotics were administered by themselves (80%). A total of 230 samples (40 each from piglets, weaners and adults, 30 each of feed, water, hand swabs and 20 boot socks) from 10 pig farms were screened and 366 E. coli isolates were used for further characterization. The average E. coli count ranged from 5.811 to 6.282 log10 cfu / g. The highest per cent of AMR E. coli counts as proportion of total E. coli was observed in kitchen waste (66.16%) followed by water trough (55.55%), human hand swabs (52.94%), outside boot socks (50.87%) and piglet (45.37%) samples as compared to other samples in this study. The overall occurrence of E. coli, tetracycline, fluoroquinolone and ESBL resistant E. coli in the entire pork production chain was 75.65, 63.48, 46.52 and 20.87 per cent, respectively. Of the 366 isolates, 355 isolates (96.99%) carried either one or the other gene, whereas, 3.01 per cent of the isolates did not harbor any of the genes screened. Majority of the isolates carried tetA (97.54%), followed by qnrB (89.34%), sul1 (82.791%), blaCTX-M (42.08%), cmlA1 (13.11%) gene and none of the isolates carried colistin resistance gene (mcr1 to mcr5). Antimicrobial susceptibility testing revealed that majority of the isolates showed resistance to tetracycline (93.14-95.19%) followed by fluoroquinolones and complete sensitivity to carbapenem followed by aminoglycosides. It was observed that 84.91 per cent of E. coli isolates were MDR and PFGE analysis revealed a high genetic diversity at 80 per cent similarity indicating that dissemination of AMR genes within the farm premises. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810206020 | |
| dc.language.iso | English | |
| dc.pages | 225 | |
| dc.publisher | KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR | |
| dc.sub | Veterinary Microbiology | |
| dc.theme | Veterinary Microbiology | |
| dc.these.type | Ph.D | |
| dc.title | ISOLATION AND MOLECULAR CHARACTERIZATION OF ANTIMICROBIAL RESISTANT Escherichia coli IN PORK PRODUCTION CHAIN IN AND AROUND BENGALURU | |
| dc.type | Thesis |