Detection and molecular characterization of food borne pathogens from ready to eat meat and milk products

Simple item page
dc.contributor.authorShathish Sharma, BJ
dc.contributor.authorSaranya, S
dc.contributor.authorRathnapraba, S
dc.contributor.authorMeignanalakshmi, S; et al.
dc.contributor.authorTANUVAS
dc.date.accessioned2020-03-06T10:49:42Z
dc.date.available2020-03-06T10:49:42Z
dc.date.issued2020-02
dc.descriptionTNV_20thMVC_PP_Feb-2020_PA06en_US
dc.description.abstractFoodborne illness still remains a major threat to public health. Among the thirty different pathogens causing food borne outbreaks, Salmonella sps, Stapylococcus aureus, Bacillus cereus, Campylobacter sps have been incriminated for large majority of outbreak cases. Now – a – days consumption of minimally processed ready to eat (RTE) food products increases the risk of food borne diseases. The aim of the present investigation was to detect the presence of FSSAI notified six food pathogens from ready to eat food products. A total of 250 samples (including milk, milk products, meat and meat products) were screened for the presence of food-borne bacterial pathogens like Staphylococcus aureus, Bacillus cereus, Campylobacter jejuni, Streptococcus sp and Salmonella sp on selective medium. Further, culture positive colonies were screened by colony morphology, gram staining, biochemical analysis and molecular methods. Phylogenetic analysis was performed for the 13 isolates including 8 isolates of Staphylococcus aureus, 5 isolates of Bacillus cereus and 3 isolates of Streptococcus. A total of 11 Staphylococcus aureus and 5 Bacillus cereus isolates from meat products were sequence analysed and submitted to Genbank. Antibiotic susceptibility test was performed for all isolates of Staphylococcus aureus, Bacillus cereus and Streptococcus spp. The results revealed that the Staphylococcus aureus had 100% resistant against Vancomycin, Methicillin and Penicillin antibiotics andBacillus cereus isolates had 100% resistant against Vancomycin and Penicillin antibiotics and 100% sensitive towards Methicillin. Whereas the Streptococcus sp. shows 100% resistant against Gentamicin, Streptomycin, Tetracycline and Ciprofloxacin. Hence, these results indicate that the ready to eat food products can act as significant source of antibiotic resistance microbial strains.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810144614
dc.keywords20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVASen_US
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleDetection and molecular characterization of food borne pathogens from ready to eat meat and milk productsen_US
dc.title.alternativeNational Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safetyen_US
dc.typePresentationen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
TNV_20thMVC_PP_Feb-2020_PA06.pdf
Size:
133.27 KB
Format:
Adobe Portable Document Format
Description:
TNV_20thMVC_PP_Feb-2020_PA06
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Poster Presentations