Detection and molecular characterization of food borne pathogens from ready to eat meat and milk products
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Date
2020-02
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Abstract
Foodborne illness still remains a major threat to public health. Among the thirty different pathogens
causing food borne outbreaks, Salmonella sps, Stapylococcus aureus, Bacillus cereus, Campylobacter sps
have been incriminated for large majority of outbreak cases. Now – a – days consumption of minimally
processed ready to eat (RTE) food products increases the risk of food borne diseases. The aim of the present
investigation was to detect the presence of FSSAI notified six food pathogens from ready to eat food products.
A total of 250 samples (including milk, milk products, meat and meat products) were screened for the presence
of food-borne bacterial pathogens like Staphylococcus aureus, Bacillus cereus, Campylobacter jejuni,
Streptococcus sp and Salmonella sp on selective medium. Further, culture positive colonies were screened by
colony morphology, gram staining, biochemical analysis and molecular methods. Phylogenetic analysis was
performed for the 13 isolates including 8 isolates of Staphylococcus aureus, 5 isolates of Bacillus cereus and
3 isolates of Streptococcus. A total of 11 Staphylococcus aureus and 5 Bacillus cereus isolates from meat
products were sequence analysed and submitted to Genbank. Antibiotic susceptibility test was performed
for all isolates of Staphylococcus aureus, Bacillus cereus and Streptococcus spp. The results revealed that
the Staphylococcus aureus had 100% resistant against Vancomycin, Methicillin and Penicillin antibiotics andBacillus cereus isolates had 100% resistant against Vancomycin and Penicillin antibiotics and 100% sensitive
towards Methicillin. Whereas the Streptococcus sp. shows 100% resistant against Gentamicin, Streptomycin,
Tetracycline and Ciprofloxacin. Hence, these results indicate that the ready to eat food products can act as
significant source of antibiotic resistance microbial strains.
Description
TNV_20thMVC_PP_Feb-2020_PA06
Keywords
Veterinary Science