GERMPLASM CHARACTERIZATION OF KAGZI AND ORNAMENTAL LIME USING MOLECULAR MARKERS

Simple item page
dc.contributor.advisorPANKAJ KUMAR
dc.contributor.authorGILL, KOMAL JEET
dc.date.accessioned2023-03-16T09:46:12Z
dc.date.available2023-03-16T09:46:12Z
dc.date.issued2022-11
dc.description.abstractABSTRACT In the present study, twenty-seven accessions of kagzi and ornamental lime were evaluated for morphological (based on IPGRI citrus descriptor), biochemical and molecular characterization (using RAPD, SCoT and SSR markers). Under morphological characterization, significant variation was recorded in all the studied parameters viz. leaf lamina attachment, leaf lamina shape, leaf lamina margins, fruit shape, shape of fruit base, shape of fruit apex and fruit texture, respectively. Likewise, minimal differences were also recorded in kagzi and ornamental lime accessions for biochemical studies i.e. total soluble solids (TSS), titratable acidity and TSS: TA ratio. These findings were further validated and confirmed using PCR-based molecular markers viz., RAPD, SCoT and SSR markers. Analysis of PCR products of 22 RAPD, 16 SCoT and 40 SSR primers showed 115, 74 and 64 amplicons. The polymorphic percentagewere ranged from 33.33% to 100% for RAPD and SCoT markers, while SSR markers showed 50% to 100% polymorphism among the studied accessions. Likewise, polymorphic information content (PIC) was ranged from 0.20 to 0.90 for RAPD markers, 0.41 to 0.99 for SCoT markers and 0.07 to 0.67 for SSR markers, respectively. The analysis of molecular variance (AMOVA) studiesamong 27 accessions of kagzi and ornamental lime proved the significant genetic differencesi.e. for RAPD (PhiPT = 0.267, P = 0.010); for SCoT (PhiPT = 0.351, P = 0.010) and for SSR (PhiPT = 0.107, P = 0.010), respectively. With the help of STRUCTURE software 2.3.4 and structure harvester ΔK was observed at k=2 indicating the genetic admixture between the two studied populations. Thus, the present study showed clear and distinct variation among kagzi and ornamental lime accessions. Therefore, it could be concluded that the use of RAPD, SCoT and SSRmolecular markers are efficient to identify diversity or closeness in genotypes which may contribute to speed up the breeding programs and aid in citrus crop improvementen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810195308
dc.keywordsGermplasm, characterization , kagzi , ornamental lime, molecular markersen_US
dc.language.isoEnglishen_US
dc.pages92+ien_US
dc.publisherUHF,NAUNIen_US
dc.subBiotechnologyen_US
dc.themeGermplasm, characterization , kagzi , ornamental lime, molecular markersen_US
dc.these.typeM.Scen_US
dc.titleGERMPLASM CHARACTERIZATION OF KAGZI AND ORNAMENTAL LIME USING MOLECULAR MARKERSen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
PDF M.Sc. Thesis Komaljeet Gill (BTC).pdf
Size:
24.41 MB
Format:
Adobe Portable Document Format
Description:
BIOTECHNOLOGY
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses