GERMPLASM CHARACTERIZATION OF KAGZI AND ORNAMENTAL LIME USING MOLECULAR MARKERS
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Date
2022-11
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UHF,NAUNI
Abstract
ABSTRACT
In the present study, twenty-seven accessions of kagzi and ornamental lime were evaluated
for morphological (based on IPGRI citrus descriptor), biochemical and molecular characterization
(using RAPD, SCoT and SSR markers). Under morphological characterization, significant variation
was recorded in all the studied parameters viz. leaf lamina attachment, leaf lamina shape, leaf lamina
margins, fruit shape, shape of fruit base, shape of fruit apex and fruit texture, respectively. Likewise,
minimal differences were also recorded in kagzi and ornamental lime accessions for biochemical
studies i.e. total soluble solids (TSS), titratable acidity and TSS: TA ratio. These findings were further
validated and confirmed using PCR-based molecular markers viz., RAPD, SCoT and SSR markers.
Analysis of PCR products of 22 RAPD, 16 SCoT and 40 SSR primers showed 115, 74 and 64
amplicons. The polymorphic percentagewere ranged from 33.33% to 100% for RAPD and SCoT
markers, while SSR markers showed 50% to 100% polymorphism among the studied accessions.
Likewise, polymorphic information content (PIC) was ranged from 0.20 to 0.90 for RAPD markers,
0.41 to 0.99 for SCoT markers and 0.07 to 0.67 for SSR markers, respectively. The analysis of
molecular variance (AMOVA) studiesamong 27 accessions of kagzi and ornamental lime proved the
significant genetic differencesi.e. for RAPD (PhiPT = 0.267, P = 0.010); for SCoT (PhiPT = 0.351, P
= 0.010) and for SSR (PhiPT = 0.107, P = 0.010), respectively. With the help of STRUCTURE
software 2.3.4 and structure harvester ΔK was observed at k=2 indicating the genetic admixture
between the two studied populations. Thus, the present study showed clear and distinct variation
among kagzi and ornamental lime accessions. Therefore, it could be concluded that the use of RAPD,
SCoT and SSRmolecular markers are efficient to identify diversity or closeness in genotypes which
may contribute to speed up the breeding programs and aid in citrus crop improvement