Molecular characterization and validation of molecular markers for tolerance to MYMV in Urdbean (Vigna mungo L. Hepper)

Simple item page
dc.contributor.advisorSamal, Kailas Chandra
dc.contributor.authorBEHERA, LAXMIPREEYA
dc.date.accessioned2018-09-06T10:46:48Z
dc.date.available2018-09-06T10:46:48Z
dc.date.issued2018
dc.descriptionTh-5233en_US
dc.description.abstractPulses have an imperative contribution in the world Agriculture and also considered as indispensable part of Indian Agriculture as India is the largest producer (17.82 million tons) and consumer of pulses in the world. Urdbean (Vigna mungo (L).Hepper) is the third most important pulse crops in India and its percent share in total production is 13.05% followed by Gram (41.20 %) and Tur (19.11%). It belongs to the family Leguminaceae with chromosome no (2n=2x=22) and genome size of 574 mb. It is well adapted to multiple cropping systems in the drier and warmer climates. Unfortunately, the productivity of urdbean in India and Odisha in particular is very low (441 kg/ha) mainly due to inherent genotype failures and losses due to pests and diseases. Among all production constraints yellow mosaic disease (YMD) caused by Yellow Mosaic Virus is the most severe problem contributing drastic yield reduction. MYMV belongs to the genus begomo virus and is transmitted by white-fly (Bemisia tabaci) that delivers these viruses through their proboscis in the phloem cells of the host plant, which causes significant reduction in photosynthetic and other physiological activities. For proper identification of genotypes and to develop high yielding, well adaptable and resistant genotypes, systematic collection, characterisation, evaluation and conservation of urdbean germplasm is very essential. The objective of the present study was to describe the nature and extent of genotypic variation among urdbean collections for a range of traits and validation of molecular markers associated with YMD. A total of 50 urdbean genotypes along with four check varieties (OBG-31, PU-31, Ujala, Prasad) were evaluated for several diverse traits for two cropping seasons at two different locations in Odisha. Analysis of data on various categorical descriptors revealed out of possible 59 variants, seven variants (Absence of hypocotyls anthocyanin coloration, cuneate leaf shape, long plant height, brown and mottled seed colour, drum shaped seed shape and large seed size) could not be observed in any of the genotype. The highest yield (1282 kg/ha) was recorded in the genotype ‘Kantapada Local’ followed by ‘PU-31’ (1270 kg/ha) whereas the lowest yield was recorded in ‘Pendibadi Local’ (796 kg/ha). With respect to YMD infection the genotypes were graded according to the 1-9 arbitrary scale. Majority of genotypes (64.81%) were included under highly susceptible group whereas. resistant and highly resistant genotypes percentage were found to be 1.85% and 11.11%. The cluster analysis revealed wide diversity in the Urdbean collection and grouped all the 54 genotypes into five clusters at 10.42 dis-similarity coefficient. The maximum dissimilarity (3.55) was observed between genotype pair ‘Semiliguda Local’ and ‘Sambalpur Local’. Six SSR (SSR-VR135, SSR-VR044, SSR-VR078, SSR-VR095, DMBSSR130 and DMB- SSR125) and two SCAR markers (YMV-1, MYMV-583) were employed for establishment linkage of marker with disease infestation. The cluster analysis based on DNA profile data classified 31 genotypes into seven clusters. The genotype pair ‘Prasad’ and ‘Jasipur Local’ had the maximum similarity coefficient (0.93) and the lowest similarity (0.13) was recorded between ‘Aska Local’ and ‘Bakuribiri jajpur Local’. It was also observed that the PCR analysis employing MYMV-583 marker amplified 580 bp DNA fragment only in YMD resistant genotypes. Similarly the molecular results obtained other SSR and SCAR markers confirmed consistent association of the marker with the MYMVresistant genotypes. Both phenotypic and molecular results confirmed that genotype ‘PU-31’, ‘Prasad’, ‘Puri Local-2’, ‘Jashipur local’, ‘Nabina’, ‘Monika’, VIXUR’, ‘WBG-108, and ‘WBG-26, were resistant to MYMV whereas ‘Sambalpur Local’ was found moderately resistant. These linked markers will boost the efficiency and precision of Mungbean Yellow Mosaic Virus resistance breeding in urdbean.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810072191
dc.keywordsMolecular characterization, validation, molecular markers, r tolerance, MYMV, Urdbean (Vigna mungo L. Hepper)en_US
dc.language.isoenen_US
dc.pages77en_US
dc.publisherOrissa University of Agriculture and Technology, Bhubaneswaren_US
dc.subAgricultural Biotechnologyen_US
dc.subjectnullen_US
dc.themeMolecular characterization and validation of molecular markersen_US
dc.these.typeM.Scen_US
dc.titleMolecular characterization and validation of molecular markers for tolerance to MYMV in Urdbean (Vigna mungo L. Hepper)en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
07 abt16,LAXMIPREEYA BEHERA Th-5233.pdf
Size:
2.3 MB
Format:
Adobe Portable Document Format
Description:
Molecular characterization and validation of molecular markers for tolerance to MYMV in Urdbean (Vigna mungo L. Hepper)
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Thesis