Molecular characterization and validation of molecular markers for tolerance to MYMV in Urdbean (Vigna mungo L. Hepper)
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Date
2018
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Orissa University of Agriculture and Technology, Bhubaneswar
Abstract
Pulses have an imperative contribution in the world Agriculture and also considered
as indispensable part of Indian Agriculture as India is the largest producer (17.82 million tons)
and consumer of pulses in the world. Urdbean (Vigna mungo (L).Hepper) is the third most
important pulse crops in India and its percent share in total production is 13.05% followed by
Gram (41.20 %) and Tur (19.11%). It belongs to the family Leguminaceae with chromosome
no (2n=2x=22) and genome size of 574 mb. It is well adapted to multiple cropping systems in
the drier and warmer climates. Unfortunately, the productivity of urdbean in India and Odisha
in particular is very low (441 kg/ha) mainly due to inherent genotype failures and losses due
to pests and diseases. Among all production constraints yellow mosaic disease (YMD) caused
by Yellow Mosaic Virus is the most severe problem contributing drastic yield reduction.
MYMV belongs to the genus begomo virus and is transmitted by white-fly (Bemisia tabaci)
that delivers these viruses through their proboscis in the phloem cells of the host plant, which
causes significant reduction in photosynthetic and other physiological activities. For proper
identification of genotypes and to develop high yielding, well adaptable and resistant
genotypes, systematic collection, characterisation, evaluation and conservation of urdbean
germplasm is very essential. The objective of the present study was to describe the nature and
extent of genotypic variation among urdbean collections for a range of traits and validation of
molecular markers associated with YMD. A total of 50 urdbean genotypes along with four
check varieties (OBG-31, PU-31, Ujala, Prasad) were evaluated for several diverse traits for
two cropping seasons at two different locations in Odisha. Analysis of data on various
categorical descriptors revealed out of possible 59 variants, seven variants (Absence of
hypocotyls anthocyanin coloration, cuneate leaf shape, long plant height, brown and mottled
seed colour, drum shaped seed shape and large seed size) could not be observed in any of the
genotype. The highest yield (1282 kg/ha) was recorded in the genotype ‘Kantapada Local’
followed by ‘PU-31’ (1270 kg/ha) whereas the lowest yield was recorded in ‘Pendibadi
Local’ (796 kg/ha). With respect to YMD infection the genotypes were graded according to
the 1-9 arbitrary scale. Majority of genotypes (64.81%) were included under highly
susceptible group whereas. resistant and highly resistant genotypes percentage were found to
be 1.85% and 11.11%. The cluster analysis revealed wide diversity in the Urdbean collection
and grouped all the 54 genotypes into five clusters at 10.42 dis-similarity coefficient. The
maximum dissimilarity (3.55) was observed between genotype pair ‘Semiliguda Local’ and
‘Sambalpur Local’. Six SSR (SSR-VR135, SSR-VR044, SSR-VR078, SSR-VR095, DMBSSR130
and DMB- SSR125) and two SCAR markers (YMV-1, MYMV-583) were
employed for establishment linkage of marker with disease infestation. The cluster analysis
based on DNA profile data classified 31 genotypes into seven clusters. The genotype pair
‘Prasad’ and ‘Jasipur Local’ had the maximum similarity coefficient (0.93) and the lowest
similarity (0.13) was recorded between ‘Aska Local’ and ‘Bakuribiri jajpur Local’. It was also
observed that the PCR analysis employing MYMV-583 marker amplified 580 bp DNA
fragment only in YMD resistant genotypes. Similarly the molecular results obtained other
SSR and SCAR markers confirmed consistent association of the marker with the MYMVresistant
genotypes. Both phenotypic and molecular results confirmed that genotype ‘PU-31’,
‘Prasad’, ‘Puri Local-2’, ‘Jashipur local’, ‘Nabina’, ‘Monika’, VIXUR’, ‘WBG-108, and
‘WBG-26, were resistant to MYMV whereas ‘Sambalpur Local’ was found moderately
resistant. These linked markers will boost the efficiency and precision of Mungbean Yellow
Mosaic Virus resistance breeding in urdbean.
Description
Th-5233
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