DEVELOPMENT OF DNA VACCEVE FOR AVIAN INFECTIOUS LARYNGOTRACHEITIS

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dc.contributor.advisorShoba K
dc.contributor.authorJaisree S
dc.contributor.authorTANUVAS
dc.contributor.otherRamesh A
dc.contributor.otherVijayarani K
dc.contributor.otherVairamuthu S
dc.date.accessioned2023-10-16T17:02:10Z
dc.date.available2023-10-16T17:02:10Z
dc.date.issued2021
dc.description.abstractThe present study was carried out to analyse the genetic relationship of infectious laryngotracheitis virus (ILTV) isolates and to develop DNA vaccine for avian infectious laryngotracheitis (ILT). A total of thirty three ILTV suspected samples were included in this study. Affected birds showed clinical signs like depression, gasping, nasal discharge with characteristic pump handle respiration. Haemorrhagic tracheitis and the presence caseous plug in tracheal lumen were the characteristic post mortem lesions. On histopathological examination, trachea from affected birds showed desquamation and necrosis of tracheal epithelium, syncytia, haemorrhage and fibrinous exudate, infiltration of mononuclear cells and eosinophilic intranuclear inclusion bodies. The ILTV suspected samples were screened by polymerase chain reaction (PCR) targeting partial ICP4 gene. Out of thirty three samples, sixteen yielded PCR product size of 635 bp and were found positive. Samples that were positive by PCR from a single farm were pooled and inoculated into 10-12 days old specific pathogen fi'ee embryonated chicken eggs via CAM route. Out of ten samples, isolation was successful with nine. The lesions observed on CAM include congestion and haemorrhage in first passage, thickened CAM in second passage and pock lesions.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810198993
dc.language.isoEnglish
dc.pages1-273
dc.subVeterinary Microbiology
dc.themeVeterinary Science
dc.these.typePh.D
dc.titleDEVELOPMENT OF DNA VACCEVE FOR AVIAN INFECTIOUS LARYNGOTRACHEITIS
dc.typeThesis
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