DEVELOPMENT OF DNA VACCEVE FOR AVIAN INFECTIOUS LARYNGOTRACHEITIS
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Date
2021
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Abstract
The present study was carried out to analyse the genetic
relationship of infectious laryngotracheitis virus (ILTV) isolates and to develop
DNA vaccine for avian infectious laryngotracheitis (ILT). A total of thirty three
ILTV suspected samples were included in this study. Affected birds showed
clinical signs like depression, gasping, nasal discharge with characteristic pump
handle respiration. Haemorrhagic tracheitis and the presence caseous plug in
tracheal lumen were the characteristic post mortem lesions. On histopathological
examination, trachea from affected birds showed desquamation and necrosis of
tracheal epithelium, syncytia, haemorrhage and fibrinous exudate, infiltration of
mononuclear cells and eosinophilic intranuclear inclusion bodies. The ILTV
suspected samples were screened by polymerase chain reaction (PCR) targeting
partial ICP4 gene. Out of thirty three samples, sixteen yielded PCR product size
of 635 bp and were found positive. Samples that were positive by PCR from a
single farm were pooled and inoculated into 10-12 days old specific pathogen fi'ee
embryonated chicken eggs via CAM route. Out of ten samples, isolation was
successful with nine. The lesions observed on CAM include congestion and
haemorrhage in first passage, thickened CAM in second passage and pock
lesions.