STUDIES ON IN VITRO PROPAGATION OF VIRUS INDEXED STRAWBERRY (Fragaria x ananassa Duch.) ITS CONSERVATION AND GENETIC FIDELITY

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dc.contributor.advisorGUPTA, RAKESH
dc.contributor.authorTHAKUR, KANIKA
dc.date.accessioned2019-08-06T08:50:30Z
dc.date.available2019-08-06T08:50:30Z
dc.date.issued2019-06
dc.description.abstractABSTRACT The present investigation on "Studies on in vitro propagation of virus indexed strawberry (Fragaria x ananassa Duch.) its conservation and genetic fidelity" was carried out at the Department of Biotechnology, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.). The survey done for TRSV in strawberry was confirmed using DAS-ELISA which revealed that no virus was reported from all the three locations (Dhaulakuan, Shimla, Solan). Since the plant material was free from virus an efficient system for micropropagation of strawberry cvs., Selva, Sweet Charlie and Brighton using runner tips was developed. The runner tips was sterilized using 0.2 per cent carbendazim for 10 min + 0.1 per cent HgCl2 for 3 minutes, which were established on MS medium consisting of 0.5 mg/l BA and 0.2 mg/l IBA. The highest multiplication of the established runner tips was obtained when MS medium supplemented with 0.5mg/l BA + 0.20 mg/l IBA + 1.0 mg/l GA3 was used. The maximum rooting of the shoots was obtained on half strength MS medium supplemented with 0.5 mg/l IBA and their maximum hardening was obtained in potting mixture consisting of cocopeat. The various conservation techniques used revealed that the survival of the runner tips at 250C was the maximum but the low temperature condition of 40C was found suitable. The encapsulation of the runner tips was done using 0.5 mg/l BA with maximum regeneration after 4 weeks of storage. Mannitol when used at the rate of 2.5 per cent resulted in 74.19 per cent survival of the encapsulated runner tips after 1 month of storage. Out of the 23 ISSR primers used 17 were informative and revealed similarity between the mother and the micropropagated plants determining the genetic uniformity of the in vitro raised plants.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810119527
dc.keywordsvitro propagation,strawberry,genetic fidelityen_US
dc.language.isoenen_US
dc.pages92+viiien_US
dc.publisherUHF,NAUNIen_US
dc.subBiotechnologyen_US
dc.subjectnullen_US
dc.themevitro propagation,strawberry,genetic fidelityen_US
dc.these.typePh.Den_US
dc.titleSTUDIES ON IN VITRO PROPAGATION OF VIRUS INDEXED STRAWBERRY (Fragaria x ananassa Duch.) ITS CONSERVATION AND GENETIC FIDELITYen_US
dc.typeThesisen_US
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