STUDIES ON IN VITRO PROPAGATION OF VIRUS INDEXED STRAWBERRY (Fragaria x ananassa Duch.) ITS CONSERVATION AND GENETIC FIDELITY
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Date
2019-06
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UHF,NAUNI
Abstract
ABSTRACT
The present investigation on "Studies on in vitro propagation of virus indexed
strawberry (Fragaria x ananassa Duch.) its conservation and genetic fidelity" was
carried out at the Department of Biotechnology, Dr Yashwant Singh Parmar University of
Horticulture and Forestry, Nauni, Solan (H.P.). The survey done for TRSV in strawberry was
confirmed using DAS-ELISA which revealed that no virus was reported from all the three
locations (Dhaulakuan, Shimla, Solan). Since the plant material was free from virus an
efficient system for micropropagation of strawberry cvs., Selva, Sweet Charlie and Brighton
using runner tips was developed. The runner tips was sterilized using 0.2 per cent
carbendazim for 10 min + 0.1 per cent HgCl2 for 3 minutes, which were established on MS
medium consisting of 0.5 mg/l BA and 0.2 mg/l IBA. The highest multiplication of the
established runner tips was obtained when MS medium supplemented with 0.5mg/l BA +
0.20 mg/l IBA + 1.0 mg/l GA3 was used. The maximum rooting of the shoots was obtained
on half strength MS medium supplemented with 0.5 mg/l IBA and their maximum hardening
was obtained in potting mixture consisting of cocopeat. The various conservation techniques
used revealed that the survival of the runner tips at 250C was the maximum but the low
temperature condition of 40C was found suitable. The encapsulation of the runner tips was
done using 0.5 mg/l BA with maximum regeneration after 4 weeks of storage. Mannitol when
used at the rate of 2.5 per cent resulted in 74.19 per cent survival of the encapsulated runner
tips after 1 month of storage. Out of the 23 ISSR primers used 17 were informative and
revealed similarity between the mother and the micropropagated plants determining the
genetic uniformity of the in vitro raised plants.
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