Isolation and characterization of genotype XIII Newcastle disease virus from Emu in India

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dc.contributor.authorGowthaman, Vasudevan
dc.contributor.authorDayal Singh, Sambhu
dc.contributor.authorDhama, Kuldeep
dc.contributor.authorAurumugam Desingu, Perumal
dc.contributor.authorKumar, Asok
dc.contributor.authorMalik, Yashpal Singh
dc.contributor.authorMunir, Muhammad
dc.contributor.authorTANUVAS
dc.date.accessioned2018-07-13T11:47:59Z
dc.date.available2018-07-13T11:47:59Z
dc.date.issued2016-06
dc.descriptionTNV_VD_2016(1-4)en_US
dc.description.abstractNewcastle disease virus (NDV) infects at least 241 species of pet and free-living birds in addition to domesticated avian species. Wild, feral and domesticated birds are recognized reservoirs of NDV, and contribute to the epidemiology of NDV in the domesticated poultry. The biological and molecular characterization of velogenic NDV (vNDV) from emus is limited. In this study, 54 tis- sues were collected from eight Emu flocks between May 2010 and January 2012 from highly poultry-dense areas of India including Namakkal, Hyderabad and Bareilly regions. The presence of vNDV was confirmed by virus isolation, fusion (F)-gene based RT-PCR, sequencing of the cleavage site and the virulence scored. One out of eight flocks received from Hyderabad region was found positive for NDV and the in vivo pathotyping revealed the isolate to be vNDV type. The sequence analysis revealed that the isolate had cleavage site of 112-R-R-R-K-R-F-117, which is typical for vNDV. Sequence and phylogenetic analysis of the partial ‘F’ gene coding regions suggested that the NDV strain belongs to genotype XIII. The Emu isolate had 98–100 % nucleotide identity with the vNDVs previously reported in poultry flocks of India. The findings of present study based on the biological and molecular characteriza- tion of Emu-origin vNDV, highlights the circulation of genotype XIII in Emus for the first time in the country. There is need to understand the possible spill over of these genetically diverse NDV strains into the commercial poultry and their possible implications in disease control strategies.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810060144
dc.keywordsNewcastle disease virus - Velogenic - Emu - Indiaen_US
dc.language.isoenen_US
dc.pages1-4en_US
dc.publisherSpringeren_US
dc.subjectVeterinary Scienceen_US
dc.titleIsolation and characterization of genotype XIII Newcastle disease virus from Emu in Indiaen_US
dc.title.alternativeVirus Diseaseen_US
dc.typeArticleen_US
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