Isolation and characterization of genotype XIII Newcastle disease virus from Emu in India
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Date
2016-06
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Newcastle disease virus (NDV) infects at least
241 species of pet and free-living birds in addition to
domesticated avian species. Wild, feral and domesticated
birds are recognized reservoirs of NDV, and contribute to
the epidemiology of NDV in the domesticated poultry. The
biological and molecular characterization of velogenic
NDV (vNDV) from emus is limited. In this study, 54 tis-
sues were collected from eight Emu flocks between May
2010 and January 2012 from highly poultry-dense areas of
India including Namakkal, Hyderabad and Bareilly
regions. The presence of vNDV was confirmed by virus
isolation, fusion (F)-gene based RT-PCR, sequencing of
the cleavage site and the virulence scored. One out of eight
flocks received from Hyderabad region was found positive
for NDV and the in vivo pathotyping revealed the isolate to
be vNDV type. The sequence analysis revealed that the
isolate had cleavage site of 112-R-R-R-K-R-F-117, which
is typical for vNDV. Sequence and phylogenetic analysis
of the partial âFâ gene coding regions suggested that the
NDV strain belongs to genotype XIII. The Emu isolate had
98â100 % nucleotide identity with the vNDVs previously
reported in poultry flocks of India. The findings of present
study based on the biological and molecular characteriza-
tion of Emu-origin vNDV, highlights the circulation of
genotype XIII in Emus for the first time in the country.
There is need to understand the possible spill over of these
genetically diverse NDV strains into the commercial
poultry and their possible implications in disease control
strategies.
Description
TNV_VD_2016(1-4)
Keywords
Veterinary Science