CLINICO-PATHOLOGICAL AND MOLECULAR DIAGNOSIS OF LUMPY SKIN DISEASE IN CATTLE
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR 585 226
Abstract
Lumpy skin disease is an infectious, emerging viral disease of cattle caused by
Lumpy skin disease virus (LSDV). The present study reports point prevalence of LSD in
cattle at Veterinary Clinical Complex, Veterinary College, Bidar in cattle was 18.85 per
cent. Based upon the course of clinical signs, early stage and late stage was recorded in
49.56 per cent and 50.44 per cent respectively. The apparent clinical signs observed were
skin nodules (100 %), lymph node enlargement (89.82 %), fever (62.39%) and edema
(46.90 %). Haematology analysis revealed leukopenia, lymphopenia, macrocytic anaemia
in early stage whereas hypochromic microcytic anaemia and thrombocytosis were
observed in late stage of the disease. Serum biochemical analysis revealed increase in AST
and ALP levels and hypoalbumenia in LSD affected cattle. Detection of LSDV infection
using polymerase chain reaction revealed, out of 26 tissue samples 17 (65.38 %) were
found positive for P32 and F gene with 192 bp and 472 bp respectively. Histopathology of
skin lesions revealed ballooning degeneration, parakeratosis and hyperkeratosis in
epidermal cell layer. Dermal cell layer was characterised by vasculitis, infarction,
thrombosis, papillary projections with downward hyperplasia and infiltration of
inflammatory cells. Intracytoplasmic round/ oval shaped eosinophilic inclusion bodies
were observed in the epithelial cells. Phylogenetic analysis revealed that the current study
isolates were having 100 per cent nuclear identity with LSDV isolates from Odisha state.