Amplification of Sheep Microsatellites Using a Modified Touch Down PCR Programme
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Date
2015-01
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Publisher
Indian Veterinary Journal
Abstract
Amplification of microsatellite loci using PCR often results in the appearance of non specific products. ‘Touchdown’ programme is a modified PCR programme that reduces co-amplification of spurious products. The article discusses the efficacy of a modified Touchdown program which enhanced the concentration of specific product, while controlling non specific amplification.