EVALUATION OF LARVAL EXTRACTS OF CHRYSOMYA MAGGOTS FOR ANTIBACTERIAL PROPERTY
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Date
2018-07
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
In the present study, the larval extracts of Chrysomya maggots was tested for
antibacterial property. The maggots were collected from wounds and carcasses in and
around Bengaluru, India. The maggots from wounds were morphologically identified as
C. bezziana and of carcasses were C. megacephala and C. albiceps. For larval extracts,
the larvae were collected from the fly colony of C. megacephala which was successfully
maintained in the laboratory, whereas larvae of C. bezziana collected from the fresh
wounds due to their obligation of oviposition on fresh wounds. About two hundred, third
instar larvae of Chrysomya species were incubated in 2 ml of ultra-pure distilled water
for one hour, centrifuged to 10,000X g and supernatant was used as excretions/ secretions
(ES). The ES and whole larval extract of C. megacephala and ES of C. bezziana larvae of
third instars were tested for antimicrobial property against Staphylococcus aureus
(MRSA and MSSA), Escherichia coli, and Pseudomonas aeruginosa by agar well
diffusion method, disc diffusion method and Minimum inhibitory concentration. The ES
of C. bezziana was not effective. Contrastingly, the ES of C. megacephala showed zone
of inhibition at the lowest concentration of 0.23 µg by Agar well diffusion method except
for P. aeruginosa but ES was found effective by disc diffusion method against all the
three bacteria. The minimum inhibitory concentration was 1.35 mg for S. aureus.
Whereas the whole larval homogenate failed to show the antimicrobial property.