Introgression of blast resistance and semi dwarfing gene sd1 in rice using marker assisted backcross breeding

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dc.contributor.advisorRathour, Rajeev
dc.contributor.authorDiliprao, Pote Tushar
dc.date.accessioned2022-02-10T07:55:18Z
dc.date.available2022-02-10T07:55:18Z
dc.date.issued2021-10-31
dc.description.abstractIn present study two blast resistance genes Pi9, Pi54 and one semi-dwarfing gene sd1 were incorporated into a tall traditional basmati rice variety, ‘Ranbir Basmati’ from donor genotypes Pusa1637 (Pi9+sd1) and DHMAS164 (Pi54) using marker-assisted backcross breeding (MABB). Molecular marker based background analysis was combined with stringent phenotypic selection to achieve a maximum of 92.63% and 96.15% recovery of recurrent parent genome in progenies of crosses Ranbir Basmati*3 /Pusa1637 and Ranbir Basmati*3 /DHMAS164 after two backcrosses. Altogether sixteen pyramid lines homozygous for three target genes (Pi9+Pi54+sd1) were identified by foreground analysis of 1219 progenies derived from the intercross of elite BC2F1 recombinants of two crosses. These 16 lines were further advanced through pedigree selection in the field to select 39 stable ICF3 lines showing phenotypic similarity to Ranbir Basmati. All the lines were found to have reconstituted the recurrent parent alleles for genes and QTLs related to aroma and amylose content. Analysis of variance revealed sufficient variation among pyramid lines for different traits except panicle length, percent spikelet fertility and thousand grain weight. As many as 14 pyramid lines viz., RPL1-11-2, RPL1-90-1, RPL1-90-2, RPL1-90-4, RPL1-262-1, RPL1- 262-2, RPL1-495-1, RPL1-495-2, RPL1-559-1, RPL1-934-2, RPL1-1075-1, RPL1-1075-3, RPL1-1086-4 and RPL1-1121-2 were found to be significantly superior to Ranbir Basmati for yield. All the pyramid lines were highly resistant to leaf and neck blast compared to recurrent parent Ranbir Basmati on which a high incidence of leaf and neck was recorded. Five high yielding semi-dwarf pyramid lines, viz., RPL1-559-1, RPL1-934-2, RPL1-1075-1, RPL1- 1075-3 and RPL1-1121-2, showing high degree of resistance to blast and superior basmati quality attributes were identified as a possible substitute to Ranbir Basmati.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810182032
dc.keywordsresistant,grain,pyramid,linesen_US
dc.language.isoEnglishen_US
dc.pages108en_US
dc.publisherPalampuren_US
dc.subAgricultural Biotechnologyen_US
dc.themeValidation of gene-derived markers for blast resistance genes Pi-9 and Pi-54,semi-dwarfing gene sd-1 in parental genotypes,Development of semi-dwarf pyramid lines of Ranbir Basmati with blast resistance genes Pi-54 and Pi-9 using marker-assisted backcross breeding and Characterization of gene pyramided lines for major agro-morphological attributes to identify superior derivatives.en_US
dc.these.typeM.Scen_US
dc.titleIntrogression of blast resistance and semi dwarfing gene sd1 in rice using marker assisted backcross breedingen_US
dc.typeThesisen_US
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