Introgression of blast resistance and semi dwarfing gene sd1 in rice using marker assisted backcross breeding
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Date
2021-10-31
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Palampur
Abstract
In present study two blast resistance genes Pi9, Pi54 and one semi-dwarfing gene sd1
were incorporated into a tall traditional basmati rice variety, ‘Ranbir Basmati’ from donor
genotypes Pusa1637 (Pi9+sd1) and DHMAS164 (Pi54) using marker-assisted backcross
breeding (MABB). Molecular marker based background analysis was combined with
stringent phenotypic selection to achieve a maximum of 92.63% and 96.15% recovery of
recurrent parent genome in progenies of crosses Ranbir Basmati*3
/Pusa1637 and Ranbir
Basmati*3
/DHMAS164 after two backcrosses. Altogether sixteen pyramid lines homozygous
for three target genes (Pi9+Pi54+sd1) were identified by foreground analysis of 1219
progenies derived from the intercross of elite BC2F1 recombinants of two crosses. These 16
lines were further advanced through pedigree selection in the field to select 39 stable ICF3
lines showing phenotypic similarity to Ranbir Basmati. All the lines were found to have
reconstituted the recurrent parent alleles for genes and QTLs related to aroma and amylose
content. Analysis of variance revealed sufficient variation among pyramid lines for different
traits except panicle length, percent spikelet fertility and thousand grain weight. As many as
14 pyramid lines viz., RPL1-11-2, RPL1-90-1, RPL1-90-2, RPL1-90-4, RPL1-262-1, RPL1-
262-2, RPL1-495-1, RPL1-495-2, RPL1-559-1, RPL1-934-2, RPL1-1075-1, RPL1-1075-3,
RPL1-1086-4 and RPL1-1121-2 were found to be significantly superior to Ranbir Basmati for
yield. All the pyramid lines were highly resistant to leaf and neck blast compared to recurrent
parent Ranbir Basmati on which a high incidence of leaf and neck was recorded. Five high
yielding semi-dwarf pyramid lines, viz., RPL1-559-1, RPL1-934-2, RPL1-1075-1, RPL1-
1075-3 and RPL1-1121-2, showing high degree of resistance to blast and superior basmati
quality attributes were identified as a possible substitute to Ranbir Basmati.