Propagation of Chlorophytum borivilianum Sant . et Fernand in vitro for conservation
| dc.contributor.advisor | Banerjee, Madhuparna | |
| dc.contributor.author | Kumari, Priyanka | |
| dc.date.accessioned | 2017-06-20T07:03:24Z | |
| dc.date.available | 2017-06-20T07:03:24Z | |
| dc.date.issued | 2014 | |
| dc.description | Propagation of Chlorophytum borivilianum Sant . et Fernand in vitro for conservation | en_US |
| dc.description.abstract | An efficient protocol for rapid in vitro propagation of valuable medicinal plant Chlorophytum borivilianum by using shoot tip as explants were done. This plant is used in the traditional medicinal system for enhancing male potency, curative for physical weakness, natal and post natal problems, remedy for diabetes and arthritis. It is an aphrodisiacs agent used for preparation of general sex tonic, and immunity-improving drug. The in vitro plantlets production system was investigated on Murashige and Skoog medium with different concentrations and combinations of different hormones such as BAP alone and in combination with AdSO4, with and without IAA and KIN for shoot multiplication. Shoot multiplication was induced on shoot tip. Multiplication was best (88.83%) in shoot tip explants when grown on MS medium supplemented with BAP (6.0 mg/l). Rooting on excised shootlets was highest (91.31%) in MS medium supplemented with 2.0 mg/l IBA along with 2.0 mg/l IAA. Rooted plantlets were transferred to the portray for hardening. The potting mix used was sterile coco peat. After plantation the portrays were kept under poly tunnel to retain almost 100% humidity. After 15 days, the new leaves and roots came out and the plantlets were transferred to poly bags containing soil: sand: FYM in 1:1:1 proportion and kept under the shade in green house for secondary hardening. The survival percentage in green house was found 95.83% . Transferred safed musli plantlets established better under net house condition compare to direct exposure into the field. Micro propagation seems to be the best method compared with other methods of multiplication. In Safed musli in vitro method increased the rate of multiplication while combating the problem of long tuber dormancy to facilitate for growing more than one crop per year. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810022036 | |
| dc.keywords | Propagation of Chlorophytum borivilianum Sant . et Fernand in vitro for conservation | en_US |
| dc.language.iso | en_US | en_US |
| dc.pages | 44 | en_US |
| dc.publisher | Birsa Agricultural University, Kanke, Ranchi, Jharkhand | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Propagation of Chlorophytum borivilianum Sant . et Fernand in vitro for conservation | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Propagation of Chlorophytum borivilianum Sant . et Fernand in vitro for conservation | en_US |
| dc.type | Thesis | en_US |