CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) AGAINST SOIL BORNE FUNGAL PATHOGENS INFECTING CHILLI 3679
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Date
2023-05
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JAU,JUNAGADH
Abstract
Chilli (Capsicum annuum L.) is one of the most important vegetable as well as
spice and a cash crop of India which is priced for its pungency. Chilli is consider as one
of the major crop of Saurashtra and major soil borne diseases viz., wilt and collar rot attack
at different stages of crop growth and reduced the yield significantly. Therefore, a
systematic study was undertaken to isolate native strains of rhizobacteria from major chilli
growing areas of Saurashtra regions in order to test their efficacy, their mechanisms of
bio control and assessment of plant growth promotion. The experiments were carried out
from 2022-23 at the Department of Plant Pathology, College of Agriculture, Junagadh
Agricultural University, Junagadh.
A total 42 rhizosphere soil samples along with chilli roots were collected from the
chilli fields of Saurashtra regions for the isolation of efficient PGPR strains. Total 60
PGPR isolates were isolated by serial dilution method on Bacillus medium, Pseudomonas
Agar (For Fluorescein), Actinomycetes Agar and Nutrient Agar media. Further
identification based on gram staining, morphological and cultural characteristics and then
biochemical characterization were done by using HiAssorted Biochemical test kit for
gram positive bacteria (KB013) and HiAssorted Biochemical test kit for gram negative
bacteria (KB002) were used for identification. Based on morphological and biochemical
charcterization, 13 isolates of Pseudomonas spp., 28 isolates of Bacillus spp. and 19
isolates of Actinomycetes spp., which belongs to genera Streptomyces were identified.