IDENTIFICATION OF DNA MARKERS LINKED TO BACTERIAL BLIGHT DISEASE IN POMEGRANATE (Punica granatum L.)
| dc.contributor.advisor | RAVISHANKAR, K. V. | |
| dc.contributor.author | AVINASH, K. N. | |
| dc.date.accessioned | 2017-06-28T03:51:15Z | |
| dc.date.available | 2017-06-28T03:51:15Z | |
| dc.date.issued | 2009-07-15 | |
| dc.description.abstract | Pomegranate (Punica granatum L.) is one of the oldest known edible fruit of tropical and subtropical regions belongs to the family Punicaceae. Bacterial blight caused by Xanthomonas axonopodis pv. punicae is one of the severe disease limiting crop yield and productivity thus, affecting the cultivation. In Indian Institute of Horticultural Research, Bangalore, attempts are being made to incorporate resistance to popular cultivars through plant breeding methods. ‘Ganesh’ is a popular variety (susceptible to bacterial blight disease) and ‘Daru’ which is a resistant genotypes are being used as parents in breeding programme. A total of 80 F2 populations derived from a cross between ‘Ganesh’ and ‘Daru’ were used to identify molecular markers linked with the resistant trait. Initially we screened ISSR (Inter Simple Sequence Repeats), RAPD (Randomly Amplified Length Polymorphism), SRAP (Sequence Related Length Polymorphism), RGA (Resistance Gene Analogues) and a few designed primers for determining polymorphism among the contrasting genotypes. A total of 41 molecular markers, including 40 RAPD and one ISSR markers were selected. These primers were then used to amplify segregating F2 s from cross `Ganesh x Daru` to develop a preliminary linkage map in Punica granatum L. using Haldane mapping function. In linkage analysis, 35 markers were mapped on 8 linkage groups. The linkage map length varied from 3.3 cM to 39.8 cM. The map covered a total length of 146.3 cM with an average marker density of 24.38 cM between the two adjacent markers. The maximum number of markers, 8 were found on the linkage groups LG2 and LG6. This linkage map developed forms a basis for development of high density mapping in pomegranate. An attempt was also made to identify marker linked to resistance by following Bulk Segregant Analysis. The primer OPD11 amplified band of approximately 1kbp size in resistant bulk and resistant parent `Daru`. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810023550 | |
| dc.language.iso | en | en_US |
| dc.pages | 122 | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | IDENTIFICATION OF DNA MARKERS LINKED TO BACTERIAL BLIGHT DISEASE IN POMEGRANATE | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | IDENTIFICATION OF DNA MARKERS LINKED TO BACTERIAL BLIGHT DISEASE IN POMEGRANATE (Punica granatum L.) | en_US |
| dc.type | Thesis | en_US |