IDENTIFICATION OF DNA MARKERS LINKED TO BACTERIAL BLIGHT DISEASE IN POMEGRANATE (Punica granatum L.)
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Date
2009-07-15
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University of Agricultural Sciences, Bangalore
Abstract
Pomegranate (Punica granatum L.) is one of the oldest known edible fruit of
tropical and subtropical regions belongs to the family Punicaceae. Bacterial blight
caused by Xanthomonas axonopodis pv. punicae is one of the severe disease limiting
crop yield and productivity thus, affecting the cultivation. In Indian Institute of
Horticultural Research, Bangalore, attempts are being made to incorporate resistance to
popular cultivars through plant breeding methods. ‘Ganesh’ is a popular variety
(susceptible to bacterial blight disease) and ‘Daru’ which is a resistant genotypes are
being used as parents in breeding programme. A total of 80 F2 populations derived from
a cross between ‘Ganesh’ and ‘Daru’ were used to identify molecular markers linked
with the resistant trait. Initially we screened ISSR (Inter Simple Sequence Repeats),
RAPD (Randomly Amplified Length Polymorphism), SRAP (Sequence Related Length
Polymorphism), RGA (Resistance Gene Analogues) and a few designed primers for
determining polymorphism among the contrasting genotypes. A total of 41 molecular
markers, including 40 RAPD and one ISSR markers were selected. These primers were
then used to amplify segregating F2 s from cross `Ganesh x Daru` to develop a
preliminary linkage map in Punica granatum L. using Haldane mapping function. In
linkage analysis, 35 markers were mapped on 8 linkage groups. The linkage map length
varied from 3.3 cM to 39.8 cM. The map covered a total length of 146.3 cM with an
average marker density of 24.38 cM between the two adjacent markers. The maximum
number of markers, 8 were found on the linkage groups LG2 and LG6. This linkage
map developed forms a basis for development of high density mapping in pomegranate.
An attempt was also made to identify marker linked to resistance by following Bulk
Segregant Analysis. The primer OPD11 amplified band of approximately 1kbp size in
resistant bulk and resistant parent `Daru`.
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