Effect of Melatonin and Canthaxanthin on cooled storage of Magra ram semen

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dc.contributor.advisorDr. J.S.Mehta
dc.contributor.authorShivendra kumar Bhalothia
dc.date.accessioned2022-03-11T10:54:55Z
dc.date.available2022-03-11T10:54:55Z
dc.date.issued2021
dc.description.abstractThe present study was conducted to investigate the effects of Melatonin (MLT) and Canthaxanthin (CX) supplementation on liquid storage of Magra ram spermatozoa. Total 48 ejaculates were collected from eight Magra rams (6 ejaculate from each ram) aged between 1.5 to 3 years reared at ICAR, CSWRI-ARC, Bikaner under uniform management conditions. The ejaculates were evaluated macroscopic for colour, consistency, total ejaculate volume, pH and microscopic for mass motility, individual progressive motility, sperm concentration, sperm viability and sperm abnormalities. The ejaculates of each collection session were pooled and were split into three aliquots (C, MLT and CX). All the aliquots were diluted (1:10) with Tris-citric egg yolk extender (TCEY). The aliquot C was devoid of antioxidant to serve as control whereas, the other two aliquots, MLT and CX, were supplemented with 1mM Melatonin and 25μM canthaxanthin, respectively. Thereafter, the samples were stored at 4oC for 72 hrs and various seminal parameters (individual progressive motility, HOST, sperm viability, sperm abnormalities) along with oxidative stress parameters like Total antioxidant capacity (TAOC) and Malondialdehyde level (MDA) were quantified at 0, 24, 48 and 72 hrs of preservation. The results indicated that the mean percentage values for progressive sperm motility, sperm viability and TAOC were significantly higher (p<0.05) in MLT treated group and HOST significantly higher in CX group. Significantly lower percentage of total sperm abnormalities and MDA level were observed in treatment groups compared to control group. It was concluded that MLT and CX supplementation improved the liquid storage capacity of Magra rams spermatozoa.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810183329
dc.language.isoEnglishen_US
dc.pages122en_US
dc.publisherRajasthan University of Veterinary and Animal Sciences, Bikaneren_US
dc.subAnimal Reproduction, Gynaecology and Obstetricsen_US
dc.themeEffect of Melatonin and Canthaxanthin on cooled storage of Magra ram semenen_US
dc.these.typeM.V.Sc.en_US
dc.titleEffect of Melatonin and Canthaxanthin on cooled storage of Magra ram semenen_US
dc.typeThesisen_US
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