“STUDIES ON CRYOPRESERVATION AND POSTTHAWED ASSESMENT OF CHHOTANAGPURI RAM SEMEN”
Loading...
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
The studies were carried out on seminal ejaculate characteristics and its cryopreservation of
Chhotanagpuri rams. The main purpose of this study was to determine the cryotolerance of
Chhotanagpuri ram spermatozoa on the basis of post-thaw semen quality. The results obtained
have been discussed earlier in detail, the same have been summarized as follows:
Seminal characters at pre- freeze stage:
1. The colour of Chhotanagpuri ram semen was found to be creamy or creamy white.
2. The overall mean ejaculate volume, mass motility, sperm concentration and live sperm
percentage were found to be 0.56±0.02 ml, 4.28±0.08, 4210.42±29.00 millions/ml and
84.50±0.65 percent respectively.
3. The overall mean value of intact plasma membrane integrity, intact acrosome status and
intact DNA integrity were found to be 78.11±0.63 percent, 83.42±0.75 percent and
89.97±0.38 percent respectively.
4. Mean live percentage, intact plasma membrane integrity, intact acrosome, and intact DNA
integrity percentage were significantly higher in neat semen samples than frozen thawed
semen.
5. The ejaculate volume was negatively and non significantly correlated with sperm
concentration, acrosome status and DNA integrity .The ejaculate volume was significantly
(P<0.05) and positively correlated with mass motility. It was positively but non
significantly correlated with live sperm % and plasma membrane integrity.
6. The mass motility was significantly (P<0.01) and positively correlated with sperm
concentration, live sperm percentage. Its correlation with acrosome status, plasma
membrane integrity and DNA integrity was positive but non significant.
7. The sperm concentration was highly significantly (P<0.01) and positively correlated with
live sperm percentage whereas it was positively and non significantly correlated with DNA
integrity. It was negatively and non significantly correlated with plasma membrane
integrity and acrosome status.
8. The live sperm percentage was significantly (P<0.05) and positively correlated with plasma
membrane integrity. It was positively but non significantly correlated with DNA integrity
and acrosome status.
9. The plasma membrane integrity was non significantly and positively correlated with
acrosome status and DNA integrity.
10. The acrosome status was positively and non significantly correlated with DNA integrity.
Post- thaw characters:
11. The overall mean value of semen progressive motility and live proportion were found to be
46.33±0.36 percent and 61.53±0.76 percent respectively. The values did not vary
significantly between the rams.
12. The overall mean value of intact plasma membrane integrity, intact acrosome status, and
intact DNA integrity were found to be 69.66±0.67 percent, 70.94±0.79 percent and
74.58±0.79 percent respectively which did not differ significantly between the rams.
13. The difference between the ejaculate volume, mass motility, sperm concentration, live
sperm percentage, plasma membrane integrity, acrosome status, and DNA integrity were
found to be non significant among the rams.
14. The plasma membrane integrity was non significantly and positively correlated with
acrosome status, live proportion, DNA integrity and also with progressive motility
percentage.
15. The acrosome status was positively and non significantly correlated with live proportion,
progressive motility percentage and DNA integrity.
16. The DNA integrity was positively and non significantly correlated with live proportion, but
non significantly and negatively correlated with progressive motility percentage.
17. The live proportion was positively and non significantly correlated with progressive
motility.
Description
“STUDIES ON CRYOPRESERVATION AND POSTTHAWED ASSESMENT OF CHHOTANAGPURI RAM SEMEN”
Keywords
null