Biotyping and Serotyping of Bacillus Cereus and Serologic Method for Detection of Enterotoxin in Food and Faec al Specimens
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Date
1992
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Birsa Agricultural University, Ranchi
Abstract
Bacillus cereus is commonly associated with food poisoning outbreaks in England, Australia, Canada, Japan and other European countries. Both 'emetic as well as diarrhoeal type of food borne illness have been reported in these countries. It has been found to be associated with diarrhoeal cases in human beings in India.
A number of B. cereus strains (averaging 50.0% of European and 100.0% of Indian) are non-typable with British serotyping system. Therefore suitable markers are needed for epidemiological studies.
Keeping this in view altogether 42 isolates of B cereus were studied. Of these 40 were isolated in this laboratory and 2 were obtained from PHLS, Colindale, England. These latter were associated with diarrhoeal and emetic type of food borne 111ness. Out of these 40 Indian isolates 26 were non-typable with British serotyping scheme while rest 14 were not sent for typing. The PHLS, Colindale, further reported that majority of these fermented xylose, a rare character and these resembled clusters of emetic type enterotoxin producers.
All 42 isolates in this study were subjected to 11 tests required for species identification. In addition 18/40 sugars used by the PHLS, Colindale were used to study fermentative ability. The isolates under test were found variable (1.e. 20-80% of strains) with respect to fermentation of xylose, salicin and cellobiose.