Biotechnological approaches for management of plant virus(es) in tomato (Solanum lycopersicum L.)

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dc.contributor.advisorBHARDWAJ, S.V.
dc.contributor.authorSIRCAIK, SHABNAM
dc.date.accessioned2016-12-26T13:00:14Z
dc.date.available2016-12-26T13:00:14Z
dc.date.issued2013
dc.description.abstractABSTRACT Present investigations entitled “Biotechnological approaches for management plant virus(es) in tomato (Solanum lycopersicum L.)” were undertaken to retrieve virus tested tomato through meristem tip culture and through use of inhibitors of plant origin. Tomato was selected as a test crop due to presence of prominent symptoms on plants (mosaic and necrosis on leaves) and their wide susceptibility to virus infection. Infected plants were collected and maintained under glass house conditions. These plants were indexed both biologically and serologically and found to be infected with PVMV. The virus cultures were also maintained under in vitro conditions by culturing apical and nodal segments from infected plants as explants on MS medium. The most suitable medium for establishment and shoot multiplication was found to be MS medium supplemented with 0.5 mg l-1 BAP. For root regeneration, half strength MS medium supplemented with 0.1 % activated charcoal and 1.0 mg l-1 NAA was found to be the best. Different sizes of meristem ranging between 0.1-1.0 mm were cultured on standardized MS medium with 0.1 mg l-1 BAP and 0.1 mg l-1 GA3. Survival of meristem and virus elimination was seen to be directly and indirectly proportional to the size of meristems. The virus was not detected in plants raised from meristem sizes ranging between 0.1- 0.4 mm. Three plants extracts (i.e Vitex negundo Linn., Prinsepia utilis Royle. and Tinospora cordifolia Willd.) at various concentrations were tried in vitro and in vivo conditions to retrieve virus free plants of tomato. Under in vitro conditions explants were found to be unable to grow on media charged with Vitex extract and browning of medium was observed whereas, the other two extracts have eliminated the virus at concentration 20 mg l-1. The extracts different concentrations (i.e. 5, 10, 15, 20 mg l-1 for spraying and 50, 100, 150, 200 mg l-1) were also tested under in vivo conditions for inhibition of the test virus and it was observed that on spraying Vitex and Tinospora extract inhibited the test virus at every concentration whereas, Prinsepia extract was not able to inhibit/eliminate the test virus. Only Vitex extract has retained its virus inhibiting properties on drenching.en_US
dc.identifier.other47895
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/92878
dc.language.isoenen_US
dc.subBiotechnologyen_US
dc.these.typeM.Sc
dc.titleBiotechnological approaches for management of plant virus(es) in tomato (Solanum lycopersicum L.)en_US
dc.typeThesisen_US
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