Biotechnological approaches for management of plant virus(es) in tomato (Solanum lycopersicum L.)
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Date
2013
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ABSTRACT
Present investigations entitled “Biotechnological approaches for management plant
virus(es) in tomato (Solanum lycopersicum L.)” were undertaken to retrieve virus tested tomato
through meristem tip culture and through use of inhibitors of plant origin. Tomato was selected as
a test crop due to presence of prominent symptoms on plants (mosaic and necrosis on leaves) and
their wide susceptibility to virus infection. Infected plants were collected and maintained under
glass house conditions. These plants were indexed both biologically and serologically and found
to be infected with PVMV. The virus cultures were also maintained under in vitro conditions by
culturing apical and nodal segments from infected plants as explants on MS medium. The most
suitable medium for establishment and shoot multiplication was found to be MS medium
supplemented with 0.5 mg l-1 BAP. For root regeneration, half strength MS medium supplemented
with 0.1 % activated charcoal and 1.0 mg l-1 NAA was found to be the best. Different sizes of
meristem ranging between 0.1-1.0 mm were cultured on standardized MS medium with 0.1 mg l-1
BAP and 0.1 mg l-1 GA3. Survival of meristem and virus elimination was seen to be directly and
indirectly proportional to the size of meristems. The virus was not detected in plants raised from
meristem sizes ranging between 0.1- 0.4 mm. Three plants extracts (i.e Vitex negundo Linn.,
Prinsepia utilis Royle. and Tinospora cordifolia Willd.) at various concentrations were tried in
vitro and in vivo conditions to retrieve virus free plants of tomato. Under in vitro conditions
explants were found to be unable to grow on media charged with Vitex extract and browning of
medium was observed whereas, the other two extracts have eliminated the virus at concentration
20 mg l-1. The extracts different concentrations (i.e. 5, 10, 15, 20 mg l-1 for spraying and 50, 100,
150, 200 mg l-1) were also tested under in vivo conditions for inhibition of the test virus and it was
observed that on spraying Vitex and Tinospora extract inhibited the test virus at every
concentration whereas, Prinsepia extract was not able to inhibit/eliminate the test virus. Only
Vitex extract has retained its virus inhibiting properties on drenching.