MARKER ASSISTED SELECTION FOR DROUGHT TOLERANCE AND EFFICIENT PHOSPHOROUS UPTAKE IN RICE (O. sativa L.)
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Date
2019
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Birsa Agricultural University
Abstract
The present investigation consisted of 371 F2 and 899 F3 segregants of the cross
(Sahbhagidhan and BPT-5204 Sub-1. Sahbhagidhan possessed phosphorus uptake and drought
tolerance genes whereas; BPT-5204 Sub-1 was negative for phosphorus uptake and susceptible
to drought conditions. Thirty five F2 segregants were subjected to molecular study at Indian
Institute of Agricultural Biotechnology (IIAB) Lab, Namkum, Ranchi to access the presence of
drought tolerance, phosphorus uptake and submergence tolerance genes. Sixteen SSR markers
were employed for checking the polymorphism between the parents. The SSR markers employed
for drought tolerance were RM 279, RM 327, RM 212, RM264, RM286 and RM5443. However,
the markers studied for phosphorus uptake were K-20-1-1 and PUP 1-K-59. Six QTLs (Y1.1,
qDTY1.1, qLT2, qTR8, qSTN11 and qSLD11) for drought tolerance and single QTL (Pup-1) for
phosphorus uptake were studied. Out of the 16 markers studied, eight markers displayed
polymorphism. However, none of the Sub-1markers displayed polymorphism and hence,
withdrawn for further studies. 14 F3 families which consisted of 88 F3 plants were homozygous
for both the genes. However, 7 F2 plants were found homozygous for both the genes and twelve
F2 plants were found homozygous for drought tolerance QTLs and only 3 F2 plants displayed
homozygosity for Pup-1 gene. Phenotypic observations were recorded for all the F2 and F3
segregants at Rice Research Farm of Department of Genetics and Plant Breeding, BAU, Kanke,
Ranchi during Kharif 2018 for the traits viz. seed yield per plant, days to flowering, plant height,
spikelets per plant, spikelets per panicle, grains per plant, grains per panicle, fertility percent,
tillers per plant, number of panicles per plant, panicle length, grain length, grain width, L/B ratio,
100 seed weight and husk colour in order to estimate various genetic parameters, namely, genetic
variability, heritability, genetic advance, correlation coefficient and path analysis.
Days to flowering, plant height, spikelets per panicle, grains per panicle, grain width and
100 seed weight displayed little GCV and PCV in F2 generation which indicated little
environmental influence on the expression of these characters. Whereas, in F3 generation GCV
and PCV increased which may be due to breakage of linkage between genes with advancement
of generation. F2 segregants possessed high heritability coupled with high genetic advance as
percent of mean for the characters viz. plant height, spikelets per panicle and grains per panicle
indicating preponderance of additive gene action and thus selection may be effective for these
traits. However, F3 segregants displayed high heritability coupled with high genetic advance as
percent of mean for plant height, number of tillers per plant, spikelets per panicle and grains per
panicle.
Description
MARKER ASSISTED SELECTION FOR DROUGHT TOLERANCE AND EFFICIENT PHOSPHOROUS UPTAKE IN RICE (O. sativa L.)