EVALUATION OF PUTATIVE POLYMORPHISM IN GENES UNDERLYING THE NATURAL RESISTANCE AGAINST BOVINE BRUCELLOSIS

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dc.contributor.advisorSUDHAKAR .K (MAJOR)
dc.contributor.advisorVINOO .R
dc.contributor.advisorVENKATA SESHAIAH .CH
dc.contributor.advisorSRINIVASA RAO .T
dc.contributor.authorCHANDANA YADAGIRI
dc.date.accessioned2024-07-04T11:46:21Z
dc.date.available2024-07-04T11:46:21Z
dc.date.issued2023-04
dc.description.abstractBrucellosis, caused by Brucella abortus is a disease of livestock that is of major economic importance worldwide, as well as zoonotic. A total of 362 samples were collected from Murrah, Holstein Friesian crossbred, Jersey crossbred, Ongole, Gir and Punganur maintained at different organised farms in Andhra Pradesh to determine the polymorphism of Solute carrier family 11-member 1 (SLC11A1) and Receptor Protein Tyrosine Phosphatase (PTPRT) genes and to investigate their association with resistance or susceptibility to bovine brucellosis. The PCR-RFLP analysis on SLC11A1 (rs109453173) with PstI on 374 bp amplicon revealed all three genotypes in the genetic groups studied, except Punganur where fixation of C allele was noticed. The higher frequency of C allele was observed in HF crossbred, Jersey crossbred and Ongole whereas higher frequency of T allele was noticed in Gir and Murrah. PTPRT/BglII (rs110465273) digestion revealed all three genotypes with higher frequency of T allele in all the genetic groups except Jersey crossbred where the frequency of C and T alleles were similar. PTPRT/DpnI (rs132841890) genotyping revealed that all the genetic groups were monomorphic with C allele. At SLC11A1/PstI locus, moderate genetic diversity in cattle genetic groups and low in Murrah buffaloes were noticed. At PTPRT/BglII locus, moderate level of genetic diversity with heterozygote excess was noticed in Murrah buffaloes and cattle genetic groups except in Ongole cattle. In a total of 222 sampled for case-control association by conducting RBPT and whose results were confirmed by iELISA 41 samples were confirmed as cases based upon the Odds Ratio (OR) values. Among cases, Murrah buffaloes and crossbred cattle were monomorphic at SLC11A1/PstI locus with GG and CC genotypes whereas in indigenous cattle all the three genotypes were observed with high frequency of GG. At PTPRT/BglII locus, none of the animal had CC genotype in cases. In Murrah buffaloes and crossbred cattle, a higher frequency of TT genotype was noticed whereas it was CT in indigenous cattle, The data upon analysis with SNPStats revealed that SLC11A1/PstI locus was non significantly (P=0.68) associated with bovine brucellosis where the odds ratio of CG versus CC-GG (overdominance inheritance model) was 0.65 (0.07-5.72) indicating that there is a heterozygote advantage and PTPRT/BglII locus was near significantly (P=0.07) associated with brucellosis with OR of TT versus CT-CC was 0.52 (0.26-1.07) suggesting TT had higher odds of bovine brucellosis (dominance model). The results from this study are suggestive of genetic predisposition for resistance against bovine brucellosis warranting studies on larger population for a valid conclusion.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810211473
dc.language.isoEnglish
dc.pages111
dc.publisherSRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
dc.subAnimal Genetics and Breeding
dc.themeEVALUATION OF PUTATIVE POLYMORPHISM IN GENES UNDERLYING THE NATURAL RESISTANCE AGAINST BOVINE BRUCELLOSIS
dc.these.typeM.V.Sc.
dc.titleEVALUATION OF PUTATIVE POLYMORPHISM IN GENES UNDERLYING THE NATURAL RESISTANCE AGAINST BOVINE BRUCELLOSIS
dc.typeThesis
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