EVALUATION OF PUTATIVE POLYMORPHISM IN GENES UNDERLYING THE NATURAL RESISTANCE AGAINST BOVINE BRUCELLOSIS
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Date
2023-04
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
Brucellosis, caused by Brucella abortus is a disease of livestock that is of major
economic importance worldwide, as well as zoonotic. A total of 362 samples were
collected from Murrah, Holstein Friesian crossbred, Jersey crossbred, Ongole, Gir and
Punganur maintained at different organised farms in Andhra Pradesh to determine the
polymorphism of Solute carrier family 11-member 1 (SLC11A1) and Receptor Protein
Tyrosine Phosphatase (PTPRT) genes and to investigate their association with resistance
or susceptibility to bovine brucellosis. The PCR-RFLP analysis on SLC11A1
(rs109453173) with PstI on 374 bp amplicon revealed all three genotypes in the genetic
groups studied, except Punganur where fixation of C allele was noticed. The higher
frequency of C allele was observed in HF crossbred, Jersey crossbred and Ongole
whereas higher frequency of T allele was noticed in Gir and Murrah. PTPRT/BglII
(rs110465273) digestion revealed all three genotypes with higher frequency of T allele
in all the genetic groups except Jersey crossbred where the frequency of C and T alleles
were similar. PTPRT/DpnI (rs132841890) genotyping revealed that all the genetic
groups were monomorphic with C allele. At SLC11A1/PstI locus, moderate genetic
diversity in cattle genetic groups and low in Murrah buffaloes were noticed. At
PTPRT/BglII locus, moderate level of genetic diversity with heterozygote excess was
noticed in Murrah buffaloes and cattle genetic groups except in Ongole cattle. In a total
of 222 sampled for case-control association by conducting RBPT and whose results were
confirmed by iELISA 41 samples were confirmed as cases based upon the Odds Ratio
(OR) values. Among cases, Murrah buffaloes and crossbred cattle were monomorphic
at SLC11A1/PstI locus with GG and CC genotypes whereas in indigenous cattle all the
three genotypes were observed with high frequency of GG. At PTPRT/BglII locus, none
of the animal had CC genotype in cases. In Murrah buffaloes and crossbred cattle, a
higher frequency of TT genotype was noticed whereas it was CT in indigenous cattle,
The data upon analysis with SNPStats revealed that SLC11A1/PstI locus was non
significantly (P=0.68) associated with bovine brucellosis where the odds ratio of CG
versus CC-GG (overdominance inheritance model) was 0.65 (0.07-5.72) indicating that
there is a heterozygote advantage and PTPRT/BglII locus was near significantly
(P=0.07) associated with brucellosis with OR of TT versus CT-CC was 0.52 (0.26-1.07)
suggesting TT had higher odds of bovine brucellosis (dominance model). The results
from this study are suggestive of genetic predisposition for resistance against bovine
brucellosis warranting studies on larger population for a valid conclusion.