PARTIAL MOLECULAR CHARACTERIZATION OF WILD AND CULTIVATED POMEGRANATE Punica granatum L.
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Date
2006
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UHF,NAUNI
Abstract
The present investigation on "Partial molecular characterization of wild and cultivated
pomegranate (Punica granatum l.)" allows us to determine the phenotypic as well as genetic variability
among pomegranate cultivars. Six pomegranate cultivars Daru (Wild pomegranate). Ganesh, G·137,
Mridula, Musket and Kandharl Kabuli were taken for characterization and evaluation. Twelve
morphological parameters were used for evaluation. Kandhari Kabuli was the best cultivars among six
regarding fruit weight (g}, seeds/fruit, seeds weight (9) and more so seed taste. Four enzymes systems
(PER, ACP, ALP and EST) were used for characterization. In RAPD study, 21 primers were used and
yielded 198 scorable bands out of which 151 were polymorphic. Thirty unique bands were observed
among 6 cultivars during the investigati\: The Jaccard's coefficient and UPGMA based analysis showed
0.62 to 0.83 similarities among these cultivars, also in cluster analysis Daru (Wild pomegranate) found to
be far away from other cultivars. Lowest value of 0.62 was found between Ganesh and G-137, whereas
maximum value of 0.83 was found between Kandhari Kabuli and Musket. This revealed that the origin of
all cultivar from same parent but long ago.
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