MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM MASTITIC MILK
Loading...
Date
2012
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
The present study was undertaken for isolation and identification
of Staphylococcus aureus obtained from cases of bovine mastitis. The
antibiotic sensitivity pattern of isolates of Staphylococcus aureus was
studied. Molecular characterization of Staphylococcus aureus was done
by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE).
For this purpose 75 milk samples were obtained from cases of
bovine clinical mastitis. These samples were received in the department
from RVC clinical complex, directly from the farmers and from organized
private dairy farms in and around Ranchi.
Out of 75 milk samples the percentage of Staphylococcus spp.
was 53.33 percent, out of which percentage of Coagulase positive
and coagualse negative Staphylococcus spp were 28% and 25.33%
respectively. The prevalence of Staphylococcal mastitis was the
highest among the mastitis caused by other bacteria.
Staphylococcus aureus and Staphylococcus epidermidis (Coagulase
negative Staphylococcus) were identified on the basis of their
cultural characteristics, morphology and staining characteristics
and biochemical profile.
The drug sensitivity test of 21 Isolates of Staphylococcus aureus
showed that Ciprofloxacin was most effective antibiotic followed by
Tetracycline, Kanamycin, Amikacin, Chloramphenicol,
Gentamicin, Streptomycin and Co-trimoxazole in decreasing order.
The organisms were moderately sensitive to Cephalexin and
Nitrofurantoin but highly resistance to Ampicillin.
Similarly, 19 isolates of Staphylococcus epidermidis were subjected
to antibiotic sensitivity test where Ciprofloxacin, Tetracycline,
amikacin, Gentamicin, Kanamycin, Chloramphenicol and Cotrimoxazole
were effective. The organisms were moderately
sensitive to Nitrofurantoin, but highly resistance to Cephalexin
and Ampicillin.
When the whole cell protein of 21 isolates of Staphylococcus
aureus were subjected to SDS PAGE against protein marker, we
got 26 protein bands range from 207.90 KDa to 6.10 KDa having
broad similarities. But 4 bands i.e. 36.35 KDa, 31.16 KDa, 24.05
KDa and 19.49 KDa were highly expressed in 8 out of 21 isolates.
When the whole cell protein of 19 isolates of Staphylococcus
epidermidis were subjected to SDS PAGE against protein marker,
we got 14 protein bands range from 239 KDa to 14.08 KDa having
broad similarities. But 2 bands i.e. 69.09 KDa and 32 KDa were
highly expressed in all the isolates.
Description
MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM MASTITIC MILK
Keywords
null