Molecular Epidemiology of Antibiotic Resistant Enterococcus spp. in Poultry and Associated Environment

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dc.contributor.advisorDr. Barkha Sharma
dc.contributor.authorAgnihotri, Ishta
dc.date.accessioned2024-05-07T15:25:38Z
dc.date.available2024-05-07T15:25:38Z
dc.date.issued2024-02-16
dc.description.abstractThe present study was undertaken to study the prevalence of Enterococcus species in various samples of poultry origin like poultry cloacal swab, poultry meat sample, handler’s hand swab and poultry associated environment (water, feed and environment) by cultural isolation, PCR detection, haemolytic activity, biofilm formation and antibiogram. Antibiotic profiling of Enterococcus spp. isolates was done by disc diffusion method. The prevalence of virulence genes like gelE, asa, hyl, esp and cyl in the Enterococcus isolates was done by PCR. Total 305 samples were processed for the isolation of Enterococcus spp. and the overall prevalence of Enterococcus spp. by cultural isolation was 55% (168/305). All the 168 presumptive Enterococcus isolates were confirmed as Enterococcus by targeting tuf gene (genus specific) and out of those 168 isolates, 124 isolates ( 73.81 %, 124/168) were positive for ddlE.faecalis gene (species specific gene for E.faecalis). None were found to be positive for ddlE.faecium (species specific gene for E.faecium). All the 168 Enterococcus isolates showed α haemolysis on 5% sheep blood agar, while 16.07% showed α hemolysis, 30.95% showed β hemolysis and 63.69% showed γ haemolysis on 5% horse blood agar. Out of 168 Enterococcus isolates, 66.67% isolates were biofilm producers. The prevalence of Enterococcus was found to be influenced by the type of samples, breed, sex, nutrient status of farm, hygiene status of farm, seasonal variation was statistically significant for the occurrence of bacteria. Enterococcus isolates showed high resistance toward Oxacillin and Mupirocin, whereas all the isolates were susceptible to Penicillin G and Vancomycin. This study found a high prevalence of multidrug resistance among Enterococci isolated from poultry, suggesting that these resistant bacteria and genes may be transported from food to humans and pose a significant risk to human health in the coming years. Study revealed that prevalence of gelE gene was comparatively high (40.47%) followed by asa gene (19.02%), esp gene (14.88%) and hyl gene (8.92%). None of the Enterococcus spp. isolates harbor cyl gene.
dc.identifier.citationSelf, Advisor,book, report pratical etc.
dc.identifier.otherU.P. Pandit Deen Dayal Upadhyaya pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU) Mathura Uttar Pradesh India-281001
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810208783
dc.language.isoEnglish
dc.pages154p
dc.publisherU.P. Pandit Deen Dayal Upadhyaya pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU) Mathura Uttar Pradesh India-281001
dc.relation.ispartofseriesN/A; N/A
dc.research.problemhe significant usage of antibiotics in poultry production and human medicine in India has led, to the emergence of multi drug resistant (MDR) Enterococcus strains as shown in our study. Moreover, results presented in this study indicate that the intestinal Enterococci of poultry could be the reservoir of various virulence genes, which can contaminate poultry meat. Monitoring antimicrobial resistance in Enterococcus and the appropriate use of antimicrobials in animal food production are essential for decreasing drug resistance in bacterial pathogens.
dc.subVeterinary Epidemiology and Preventive Medicine
dc.themeMolecular Epidemiology of Antibiotic Resistant Enterococcus spp. in Poultry and Associated Environment
dc.these.typeM.V.Sc.
dc.titleMolecular Epidemiology of Antibiotic Resistant Enterococcus spp. in Poultry and Associated Environment
dc.typeThesis
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