STUDIES ON COST EFFECTIVE MICROPROPAGATION AND ASSESSMENT OF GENETIC STABILITY INSaussurea costus (Kuth)

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dc.contributor.advisorSHARMA, RAJNISH
dc.contributor.authorTHAKUR, KAMAL
dc.date.accessioned2019-02-01T10:05:36Z
dc.date.available2019-02-01T10:05:36Z
dc.date.issued2018-12
dc.description.abstractABSTRACT As micropropagation technology is generally more expensive than other conventional methods of plant propagation therefore, sometimes the unit cost per plant becomes unaffordable despite its obvious advantages. It is major concern limiting its wide application. Hence, the present investigation was aimed at studies on cost effective micropropagation and assessment of genetic stability in Saussurea costus (Kuth)-an endangered medicinal herb of North-Western Himalyan region. Four different types of explants viz. cotyledon, hypocotyls, leaf and shoot tip were subjected to attempt in vitro plant regeneration. Shoot tip explant was found to be good and induced highest direct shoot regeneration i.e, 68.46% on MS medium supplemented with 0.25 mg/l TDZ and 0.50 mg/l NAA. Cost effective medium containing 45 g/l table sugar, 50 g/l starch and aquaguard water was found to be best for multiplication and rooting. Morphogenetic response of multiplication and rooting in S. costus microshoots varied significantly on cost effective media as compared to control medium. Further, randomly selected plants (from mother plants and in vitro regenerants raised on control as well as cost effective media) were subjected to assess genetic stability with the help of RAPD and ISSR markers. The molecular marker analyses revealed genetic stability by obtaining monomorphic banding profiles among all the subjected DNA samples. It was inferred from the present study that cost effective approaches are not successful in the efficient micropropagation of S. costus due to slow growth, less multiplication and high mortality rate on cost effective medium. Therefore, only standard MS medium would be preferred for in vitro propagation and conservation strategies of this high value age-old medicinal herb.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810093263
dc.keywordsSaussurea costus (Kuth), medicinal herb,cost effective micropropagation,assessment of genetic stabilityen_US
dc.language.isoenen_US
dc.pages83+iven_US
dc.publisherUHF, NAUNIen_US
dc.subBiotechnologyen_US
dc.subjectnullen_US
dc.themeSaussurea costus (Kuth), medicinal herb,cost effective micropropagation,assessment of genetic stabilityen_US
dc.these.typeM.Scen_US
dc.titleSTUDIES ON COST EFFECTIVE MICROPROPAGATION AND ASSESSMENT OF GENETIC STABILITY INSaussurea costus (Kuth)en_US
dc.typeThesisen_US
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