STUDIES ON COST EFFECTIVE MICROPROPAGATION AND ASSESSMENT OF GENETIC STABILITY INSaussurea costus (Kuth)
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Date
2018-12
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UHF, NAUNI
Abstract
ABSTRACT
As micropropagation technology is generally more expensive than other conventional
methods of plant propagation therefore, sometimes the unit cost per plant becomes unaffordable
despite its obvious advantages. It is major concern limiting its wide application. Hence, the present
investigation was aimed at studies on cost effective micropropagation and assessment of genetic
stability in Saussurea costus (Kuth)-an endangered medicinal herb of North-Western Himalyan
region. Four different types of explants viz. cotyledon, hypocotyls, leaf and shoot tip were subjected
to attempt in vitro plant regeneration. Shoot tip explant was found to be good and induced highest
direct shoot regeneration i.e, 68.46% on MS medium supplemented with 0.25 mg/l TDZ and 0.50
mg/l NAA. Cost effective medium containing 45 g/l table sugar, 50 g/l starch and aquaguard water
was found to be best for multiplication and rooting. Morphogenetic response of multiplication and
rooting in S. costus microshoots varied significantly on cost effective media as compared to control
medium. Further, randomly selected plants (from mother plants and in vitro regenerants raised on
control as well as cost effective media) were subjected to assess genetic stability with the help of
RAPD and ISSR markers. The molecular marker analyses revealed genetic stability by obtaining
monomorphic banding profiles among all the subjected DNA samples. It was inferred from the
present study that cost effective approaches are not successful in the efficient micropropagation of S.
costus due to slow growth, less multiplication and high mortality rate on cost effective medium.
Therefore, only standard MS medium would be preferred for in vitro propagation and conservation
strategies of this high value age-old medicinal herb.
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