‘Molecular insight into the protective role of green copper nanoparticle against Fusarium oxysporum in chickpea.
| dc.contributor.advisor | Dr. BIPLAB SARKAR | |
| dc.contributor.author | PRATIK PRASAD SINGH | |
| dc.contributor.other | T-11284 | |
| dc.date.accessioned | 2024-10-24T06:13:51Z | |
| dc.date.available | 2024-10-24T06:13:51Z | |
| dc.date.issued | 2023 | |
| dc.description.abstract | Copper is a potent antifungal agent for plant protection. Usually wilt disease caused by Fusarium oxysporium is considered as a unsolved paradox in the pulse’s cultivation. In the current study, the nano scale copper efficacy has been evaluated to nullify the fungal wilt infestation in the pulse crops. The synthesis of copper nanoparticles (CuO NPs) was carried out by green method and characterised by FTIR, UV-Vis and DLS. Through in- vitro study, the poisoned food technique was conducted to screen the different doses of nano-copper against the F. oxysporum. Further, selected doses (500 ppm, 750 ppm and 1000 ppm) were used in vivo model, the treatments containing 500 ppm showed the better morpho physiological outcomes which was well- supported by the histological as well as FACS evidences. It was reported that the copper responsive gene such as CHI F, CAO, SOD, GATA and LACCASE against the fungus causing wilt were upregulated in the treatment group containing 500 ppm. Hence, the CuO NPs can be an efficacious alternative to replace the conventional use of copper sulphate in wilt disease in the chickpeas. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810215818 | |
| dc.language.iso | English | |
| dc.publisher | DIVISION OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY ICAR-INDIAN INSTITUTE OF AGRICULTURAL BIOTECHNOLOGY, RANCHI- 834003 ICAR-INDIAN AGRICULTURAL RESEARCH INSTITUTE | |
| dc.sub | Biotechnology and molecular Biology | |
| dc.theme | Molecular insight into the protective role of green copper nanoparticle against Fusarium oxysporum in chickpea. | |
| dc.these.type | M.Sc | |
| dc.title | ‘Molecular insight into the protective role of green copper nanoparticle against Fusarium oxysporum in chickpea. | |
| dc.type | Thesis |