‘Molecular insight into the protective role of green copper nanoparticle against Fusarium oxysporum in chickpea.
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Date
2023
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DIVISION OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY ICAR-INDIAN INSTITUTE OF AGRICULTURAL BIOTECHNOLOGY, RANCHI- 834003 ICAR-INDIAN AGRICULTURAL RESEARCH INSTITUTE
Abstract
Copper is a potent antifungal agent for plant protection. Usually wilt disease caused by Fusarium
oxysporium is considered as a unsolved paradox in the pulse’s cultivation. In the current study, the nano
scale copper efficacy has been evaluated to nullify the fungal wilt infestation in the pulse crops. The
synthesis of copper nanoparticles (CuO NPs) was carried out by green method and characterised by
FTIR, UV-Vis and DLS. Through in- vitro study, the poisoned food technique was conducted to screen
the different doses of nano-copper against the F. oxysporum. Further, selected doses (500 ppm, 750 ppm
and 1000 ppm) were used in vivo model, the treatments containing 500 ppm showed the better morpho physiological outcomes which was well- supported by the histological as well as FACS evidences. It
was reported that the copper responsive gene such as CHI F, CAO, SOD, GATA and LACCASE against
the fungus causing wilt were upregulated in the treatment group containing 500 ppm. Hence, the CuO
NPs can be an efficacious alternative to replace the conventional use of copper sulphate in wilt disease
in the chickpeas.