DYNAMICS OF BIOCHEMICAL COMPOUNDS AND ENDOPHYTIC POPULATION IN SUSCEPTIBLE AND RESISTANT GENOTYPES OF POTATO IN RESPONSE TO LATE BLIGHT DISEASE

Simple item page
dc.contributor.authorSHRUTHILA KAKADE
dc.date.accessioned2024-03-13T11:53:34Z
dc.date.available2024-03-13T11:53:34Z
dc.date.issued2024-02-09
dc.description.abstractPresent study aimed to assess the defense responses of wild and cultivated potato genotypes against late blight (LB) through the application of defense modulators, including Jasmonic acid (JA), compost tea (CT), and salicylic acid (SA). The field experiment, conducted in Karnataka's Hassan district during Kharif-2019 and 2022, featured two wild accessions (LB resistant Solanum sparsipillium/SS-1724-07/AC4 and susceptible Solanum spegzinii/SS-1725-54/AC6) and two cultivars (LB susceptible Kufri jyoti and moderately resistant Kufri himalini). In both whole plant (field, pot) and cellular (calli) experiments, CT and JA treatments enhanced defense biochemicals compared to the control. HPLC analysis revealed increased endogenous JA in pot experiment treated with CT, suggesting potential induction of similar defense pathways by CT and JA. The defense parameters monitored such as SOD(CT: KH-9.50, KJ-4.8, AC4-20.74, AC6-5.11; JA: KH-17.75, KJ- 13.39, AC4-17.40, AC6-15.80 μg protein for 50% inhibition), POX activities (CT: KH- 350.12, KJ-375.34, AC4-347.10, AC6-322.00; JA: KH-324.67, KJ-300.18, AC4-346.00, AC6-385.00 μg min-1 mg-1 protein), phenolics (CT:, KH-10.22, KJ-10.24, AC4-11.24, AC6-11.45; JA: KH-11.53, KJ-11.13, AC4-10.14, AC6-10.11 mg GAE/100g FW), flavonoids(CT: KH-51.49, KJ- 55.28, AC4-55.78, AC6-49.01; JA: KH- 50.82, KJ-48.80, AC4-58.53, AC6-56.86 μg/100g FW) and proline levels significantly increased in CT, JA. Elevated levels of defense biochemicals without pathogen indicated a priming effect of CT and JA. Sugar levels were enhanced in diseased plants but sugar contents reduced in callus of all genotypes in the presence of Phytopthora infestans (PI) which may be a mechanism to curtail the growth of pathogen in the vicinity of infection at whole plant level. JA and CT treatments resulted in higher H2O2 accumula tion in callus cells associated with cell death/mortality validating earlier work. Endophytic characterization identified Bacillus cereus and Staphylococcus sps in tubers, with GC-MS analysis revealing useful metabolites like 5-Eicosene with antimicrobial activities. While monomorphic markers (R3a, STM 1064) appeared uninformative, they still hold value in genetic studies
dc.identifier.otherTh-14039
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810207489
dc.keywordsDYNAMICS OF BIOCHEMICAL COMPOUNDS AND ENDOPHYTIC
dc.keywordsPOPULATION IN SUSCEPTIBLE AND RESISTANT GENOTYPES OF POTATO
dc.language.isoEnglish
dc.pages299
dc.publisherUNIVERSITY OF AGRICULTURAL SCINCES BANGALORE
dc.subPlant Biotechnology
dc.subjectDYNAMICS OF BIOCHEMICAL COMPOUNDS AND ENDOPHYTIC
dc.subjectPOPULATION IN SUSCEPTIBLE AND RESISTANT GENOTYPES OF POTATO
dc.themeDYNAMICS OF BIOCHEMICAL COMPOUNDS AND ENDOPHYTIC POPULATION IN SUSCEPTIBLE AND RESISTANT GENOTYPES OF POTATO IN RESPONSE TO LATE BLIGHT DISEASE
dc.these.typePh.D
dc.titleDYNAMICS OF BIOCHEMICAL COMPOUNDS AND ENDOPHYTIC POPULATION IN SUSCEPTIBLE AND RESISTANT GENOTYPES OF POTATO IN RESPONSE TO LATE BLIGHT DISEASE
dc.typeThesis
Files
Collections
Theses