DYNAMICS OF BIOCHEMICAL COMPOUNDS AND ENDOPHYTIC POPULATION IN SUSCEPTIBLE AND RESISTANT GENOTYPES OF POTATO IN RESPONSE TO LATE BLIGHT DISEASE
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Date
2024-02-09
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UNIVERSITY OF AGRICULTURAL SCINCES BANGALORE
Abstract
Present study aimed to assess the defense responses of wild and cultivated potato genotypes against late blight (LB) through the application of defense modulators, including Jasmonic acid (JA), compost tea (CT), and salicylic acid (SA). The field experiment, conducted in Karnataka's Hassan district during Kharif-2019 and 2022, featured two wild accessions (LB resistant Solanum sparsipillium/SS-1724-07/AC4 and susceptible Solanum spegzinii/SS-1725-54/AC6) and two cultivars (LB susceptible Kufri jyoti and moderately resistant Kufri himalini). In both whole plant (field, pot) and cellular (calli) experiments, CT and JA treatments enhanced defense biochemicals compared to the control. HPLC
analysis revealed increased endogenous JA in pot experiment treated with CT, suggesting potential induction of similar defense pathways by CT and JA. The defense parameters monitored such as SOD(CT: KH-9.50, KJ-4.8, AC4-20.74, AC6-5.11; JA: KH-17.75, KJ-
13.39, AC4-17.40, AC6-15.80 μg protein for 50% inhibition), POX activities (CT: KH- 350.12, KJ-375.34, AC4-347.10, AC6-322.00; JA: KH-324.67, KJ-300.18, AC4-346.00, AC6-385.00 μg min-1 mg-1 protein), phenolics (CT:, KH-10.22, KJ-10.24, AC4-11.24, AC6-11.45; JA: KH-11.53, KJ-11.13, AC4-10.14, AC6-10.11 mg GAE/100g FW), flavonoids(CT: KH-51.49, KJ- 55.28, AC4-55.78, AC6-49.01; JA: KH- 50.82, KJ-48.80,
AC4-58.53, AC6-56.86 μg/100g FW) and proline levels significantly increased in CT, JA. Elevated levels of defense biochemicals without pathogen indicated a priming effect of CT and JA. Sugar levels were enhanced in diseased plants but sugar contents reduced in callus
of all genotypes in the presence of Phytopthora infestans (PI) which may be a mechanism to curtail the growth of pathogen in the vicinity of infection at whole plant level. JA and CT treatments resulted in higher H2O2 accumula tion in callus cells associated with cell
death/mortality validating earlier work. Endophytic characterization identified Bacillus cereus and Staphylococcus sps in tubers, with GC-MS analysis revealing useful metabolites like 5-Eicosene with antimicrobial activities. While monomorphic markers (R3a, STM 1064) appeared uninformative, they still hold value in genetic studies
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DYNAMICS OF BIOCHEMICAL COMPOUNDS AND ENDOPHYTIC, POPULATION IN SUSCEPTIBLE AND RESISTANT GENOTYPES OF POTATO