CHARACTERIZATION OF CHRYSANTHEMUM (Dendranthema grandiflora Tzvelev) GERMPLASM THROUGH MORPHOLOGICAL AND MOLECULAR MARKERS
| dc.contributor.advisor | KUMAR, D. P. | |
| dc.contributor.author | MUKUND, SHIRAGUR. | |
| dc.date.accessioned | 2017-03-14T09:40:10Z | |
| dc.date.available | 2017-03-14T09:40:10Z | |
| dc.date.issued | 2009-07-10 | |
| dc.description.abstract | Flowers and flowering plants have been a fascinating part of our life. Among them chrysanthemums are the most interesting and oldest ornamental flower crops in the world. In India, it is considered as traditional flower and used for religious offerings, garden display and for making garlands. In the recent years it is preferred particularly due to the wide range of shapes and size of flowers, brilliance of colour tones, long lasting floret life and diversity in the gene pool. Thus, an attempt was made to study genetic diversity in 42 selected chrysanthemum genotypes employing morphological and molecular markers. Based on morphological traits, it is revealed that the genotypes were not clearly grouped as separate cluster by morphological dendrogram and PCA. This may be due to less number of morphological traits considered for the study. In chrysanthemum, identification of the phenotypes is still based on morphological characters. However, many of them cannot be readily distinguished by morphological indices, particularly if they are closely related. Further more, phenotype identification based on morphological traits is subject to environmental variation. The genetic diversity of chrysanthemum, as reveled by PCA and dendrogram generated by RAPD markers, it was evident that genotypes were comparatively less diverse (11 %). This could be partly attributed that the RAPD markers could not amplify more number of loci per primer. Using nine EcoRI and MseI adapter specific primer combinations generated the AFLP fingerprint. The primer combination E- GAC/M-TAA and E- GTA/M- TCG gave the best polymorphism for most of the genotypes. The dendrogram drawn for these 42 genotypes gave grouping of various genotypes. They were grouped into two major clusters. Major cluster-I consisting of only the genotypes developed at IIHR, Bangalore and major cluster-II includes the other genotypes collected from different parts of India. The genetic distance values ranged from 17 to 114 suggesting a higher genetic variation within the chrysanthemum genotypes. The highest dissimilarity per cent was observed between the accessions ‘Fitonia’ and ‘Usha Kiran’ which were collected from distinct sources. The PCA diagram also gave clear-cut grouping of 42 germplasm. The present study clearly reflects the utility of AFLPs in fingerprinting and estimation of genetic diversity within genotypes. From the studies it was found that genetic variation among selected chrysanthemum genotypes was high. The genetic distance information can be for marker-assisted selection (MAS) and for the identification of diverse parents for use in hybridization programme. | en_US |
| dc.identifier.other | Th-9489 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810004875 | |
| dc.language.iso | en | en_US |
| dc.pages | 165 | en_US |
| dc.publisher | University of Agricultural Sciences GKVK, Bangalore | en_US |
| dc.sub | Horticulture | en_US |
| dc.subject | null | en_US |
| dc.theme | GERMPLASM THROUGH MORPHOLOGICAL | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | CHARACTERIZATION OF CHRYSANTHEMUM (Dendranthema grandiflora Tzvelev) GERMPLASM THROUGH MORPHOLOGICAL AND MOLECULAR MARKERS | en_US |
| dc.type | Thesis | en_US |