EVALUATION OF IN-VITRO ANTHELMINTIC ACTIVITY OF CURCUMIN AND PIPERINE NANOPARTICLES
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The present study was carried out with an aim to synthesize and characterize
curcumin nanoparticles and piperine nanoparticles and to evaluate the in vitro anthelmintic
potential of curcumin- and piperine-nanoparticles against gastrointestinal (GI) nematodes
of sheep. Curcumin nanoparticles and piperine nanoparticles were synthesized by using
solvent (-) anti-solvent method. Scanning electron microscopy (SEM) analysis showed the
average particle size of 32nm and 90nm for curcumin nanoparticles and piperine
nanoparticles respectively. Dynamic light scattering (DLS) analysis showed hydrodynamic
diameter of 261nm and 228nm for curcumin nanoparticles and piperine nanoparticles
respectively. The zeta potential of curcumin nanoparticles and piperine nanoparticles was
measured to be -24.9mV and -10.2mV respectively. Polydispersity index of curcumin
nanoparticles and piperine nanoparticles was measured to be 25.4 per cent and 24 per cent
respectively. Curcumin, piperine, curcumin nanoparticles and piperine nanoparticles were
investigated for in vitro anthelmintic potential with albendazole as standard of comparison,
against GI nematodes of sheep at various concentrations (0.025, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5
and 0.6 μg/mL). The in vitro anthelmintic assays employed in the present study were egg
hatch assay (EHA), larval development assay (LDA) and larval motility assay (LMA). The
piperine nanoparticles (LC50: 0.035μg/ml) showed the good anthelmintic activity in terms
of EHA compared to albendazole (LC50: 0.169μg/ml), showed the good anthelmintic
activity in terms of EHA compared to albendazole (LC50: 0.152μg/ml), curcumin (LC50:
0.214μg/ml) and curcumin nanoparticles (LC50: 0.056μg/ml) in the present study. The
piperine nanoparticles (LC50: 0.062μg/ml) showed the good anthelmintic activity in terms
of LDA compared to albendazole (LC50: 0.142μg/ml), piperine (LC50: 0.169μg/ml),
curcumin (LC50: 0.094μg/ml) and curcumin nanoparticles (LC50: 0.071μg/ml) in the
present study. The curcumin nanoparticles (LC50: 0.071μg/ml) exhibited good anthelmintic
activity in terms of LMA compared to albendazole (LC50: 0.142μg/ml) and curcumin
(LC50: 0.094μg/ml), whereas piperine and piperine nanoparticles did not show inhibitory
effect on L3 larval motility in the present study.
Key words: Curcumin nanoparticles, piperine nanoparticles, in vitro anthelmintic assays.