In vitro Regeneration of Desert Medicinal Plant Ghrit Kumari [Aloe barbadensis Mill.]

Simple item page
dc.contributor.advisorJakhar, M. L.
dc.contributor.authorSarfraz Ahmad
dc.date.accessioned2024-05-06T16:19:43Z
dc.date.available2024-05-06T16:19:43Z
dc.date.issued2022
dc.description.abstractAloe barbadensis Miller (syn. Aloe vera L.) is a succulent desert medicinal plant and has great importance in pharmaceutical and cosmetic industries. Its large scale cultivation through natural mean of shoot/ suckers is problematic due to insufficient availability. Therefore, the present investigation was carried out with the objectives for optimization of the condition of shoot proliferation, root induction, callus proliferation and regeneration of Aloe barbadensis to generate large number of planting material in short time. Effect of culture media, antioxidants, photoperiod and genotypes were also studied to enhance regeneration proficiency. Different levels of plant growth regulators (PGRs) were used for shoot proliferation, callus induction, organogenesis and root induction. The best identified PGRs were further used to study the different factors affecting the in vitro culture. The cultures were incubated at 25±2°C temperature under 14 hours light followed by 10 hours dark period with light intensity of 3000 lux. The analysis of variance showed wide variation among the different treatments/levels of PGRs for all the studied characters. For direct shoot proliferation, BAP alone at 3.5 mg/l in MS medium was found best as it showed highest shoot multiplication rate with 100 per cent regeneration frequency. Profuse and viable callus proliferation was obtained at 2.5 mg/l 2,4-D, while a combination of 2.0 mg/l Kn + 1.0 mg/l NAA was found most responsive for highest number of shoot regeneration per callus culture in shortest period. Maximum number and longest root induction was obtained at 1.5 mg/l IBA and survival rate during hardening and acclimatization process was also highest in IBA derived rooted plantlets of Aloe barbadensis. Among all the studied culture media, MS medium was found best for direct shoot proliferation, while Woody Plant Medium (WPM) was most optimum for root induction, callus proliferation and regeneration in callus. Incorporation of all the levels of activated charcoal (AC) in MS media was advantageous while PVP reduces the efficacy of micropropagation of Aloe. The identified doses of AC for maximum response in MS medium were 175 mg/l, 100 mg/l, 100 mg/l and 225 mg/l for shoot multiplication, root induction, callus proliferation and regeneration, respectively. Among different photoperiod regimes, light and dark hour‘s period of 16: 8 for direct shoot induction, 14: 10 for root multiplication and 12: 12 for callus proliferation and regeneration were found best. Genotype JA-2 showed maximum regeneration response for shooting, rooting, callusing and organogenesis while genotype JA-3 was found least responsive. The above protocol may be used for large scale multiplication of genetically uniform plants of Aloe barbadensis to minimize the cost of production.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810208727
dc.keywordsIn vitro Regeneration
dc.keywordsDesert Medicinal Plant
dc.keywordsAloe barbadensis Mill
dc.language.isoEnglish
dc.research.problemIn vitro Regeneration of Desert Medicinal Plant Ghrit Kumari [Aloe barbadensis Mill.]
dc.subGenetics and Plant Breeding
dc.themeIn vitro Regeneration of Desert Medicinal Plant Ghrit Kumari [Aloe barbadensis Mill.]
dc.these.typePh.D
dc.titleIn vitro Regeneration of Desert Medicinal Plant Ghrit Kumari [Aloe barbadensis Mill.]
dc.typeThesis
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Sarfraz Ahmad Ph.D. Thesis Plant Breeding and Genetics.pdf
Size:
5.53 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Thesis